Bactericidal efficacy of nitric oxide-releasing silica nanoparticles

被引：286

作者：

Hetrick, Evan M. ^{[1
]}

Shin, Jae Ho ^{[1
]}

Stasko, Nathan A. ^{[1
]}

Johnson, C. Bryce ^{[1
,2
]}

Wespe, Daniel A. ^{[1
]}

Holmuhamedov, Ekhson ^{[2
]}

Schoenfisch, Mark H. ^{[1
]}

机构：

[1] Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA

[2] Univ N Carolina, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USA

来源：

ACS NANO | 2008年 / 2卷 / 02期

关键词：

nitric oxide; silica nanoparticle; antibacterial; bactericidal; cytotoxicity; reactive nitrogen species; reactive oxygen species;

D O I：

10.1021/nn700191f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The utility of nitric oxide (NO)-releasing silica nanoparticles as novel antibacterial agents is demonstrated against Pseudomonas aeruginosa. Nitric oxide-releasing nanoparticles were prepared via cocondensation of tetraalkoxysilane with aminoalkoxysilane modified with diazeniumdiolate NO donors, allowing for the storage of large NO payloads. Comparison of the bactericidal efficacy of the NO-releasing nanoparticles to 1-[2-(carboxylato)pyrrolidin-1-yl]diazen-1-ium-1,2-diolate (PROLI/NO), a small molecule NO donor, demonstrated enhanced bactericidal efficacy of nanoparticle-derived NO and reduced cytotoxicity to healthy cells (mammalian fibroblasts). Confocal microscopy revealed that fluorescently labeled NO-releasing nanoparticles associated with the bacterial cells, providing rationale for the enhanced bactericidal efficacy of the nanoparticles. Intracellular NO concentrations were measurable when the NO was delivered from nanoparticles as opposed to PROLI/NO. Collectively, these results demonstrate the advantage of delivering NO via nanoparticles for antimicrobial applications.

引用

页码：235 / 246

页数：12

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