Fast and precise method for HPLC-size exclusion chromatography with UV and TOC (NDIR) detection: Importance of multiple detectors to evaluate the characteristics of dissolved organic matter

被引：44

作者：

Kawasaki, Nobuyuki ^{[1
]}

Matsushige, Kazuo ^{[1
]}

Komatsu, Kazuhiro ^{[1
]}

Kohzu, Ayato ^{[1
]}

Nara, Fumiko Watanabe ^{[2
]}

Ogishi, Fumikazu ^{[3
]}

Yahata, Masahito ^{[3
]}

Mikami, Hirohisa ^{[3
]}

Goto, Takeshi ^{[3
]}

Imai, Akio ^{[1
]}

机构：

[1] Natl Inst Environm Studies, Tsukuba, Ibaraki 3058506, Japan

[2] Tohoku Univ, Grad Sch Sci, Aoba Ku, Sendai, Miyagi 9808578, Japan

[3] Shimadzu Co Ltd, Nakagyou Ku, Kyoto 6048511, Japan

来源：

WATER RESEARCH | 2011年 / 45卷 / 18期

基金：

日本学术振兴会;

关键词：

HPLC; SEC; Apparent MW; DOM size; HUMIC SUBSTANCES; INORGANIC CARBON; MOLECULAR-WEIGHT; DOC; OXIDATION; RIVERS; WATER;

D O I：

10.1016/j.watres.2011.09.021

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A new type of high-performance liquid chromatography (HPLC) size exclusion chromatography (SEC) system with ultraviolet (UV) absorbance detection and non-dispersive infrared (NDIR) detection of total organic carbon is described. The introduction of an online degassing tube and a low-volume HPLC column helped to reduce the analytical time and increase the sensitivity of the SEC system. This study is the first in which linear calibration curves (R(2) > 0.99) were obtained for both UV absorbance and NDIR data for polystyrene sulfonate standards, which are the most suitable standards for molecular size analysis of aquatic humic substances as well as dissolved organic matter (DOM). Using the calibration curves, the molecular size distribution of DOM in water collected from Lake Kasumigaura and in pore water from lake sediments was estimated. Most of the DOM had a molecular weight less than 4000 Daltons (Da), and the amount of low-molecular-weight DOM (similar to 2000 Da) with low UV absorbance increased with depth in the sediment pore water. This result shows the importance of combining quantitative analysis by NDIR detection with qualitative analysis by UV detection to determine the chemical and physical properties of DOM. The possible sources and reactivity of DOM in Lake Kasumigaura and its sediment pore water are also discussed. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：6240 / 6248

页数：9

共 31 条

[11] THE ABUNDANCE, DISTRIBUTION, AND CONFIGURATION OF POREWATER ORGANIC COLLOIDS IN RECENT SEDIMENTS [J].

CHIN, YP ;

GSCHWEND, PM .

GEOCHIMICA ET COSMOCHIMICA ACTA, 1991, 55 (05) :1309-1317

[12] MOLECULAR-WEIGHT, POLYDISPERSITY, AND SPECTROSCOPIC PROPERTIES OF AQUATIC HUMIC SUBSTANCES [J].

CHIN, YP ;

AIKEN, G ;

OLOUGHLIN, E .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1994, 28 (11) :1853-1858

[13] High pressure size exclusion chromatography (HPSEC) of humic substances: Molecular sizes, analytical parameters, and column performance [J].

Conte, P ;

Piccolo, A .

CHEMOSPHERE, 1999, 38 (03) :517-528

[14]

Decho A.W., 1990, OCEANOGRAPHY MARINE, V28, P75

[15]

Fenchel T., 1995, ECOLOGY EVOLUTION AN

[16] Extra-cellular polysaccharides, soluble microbial products, and natural organic matter impact on nanofiltration membranes flux decline [J].

Fonseca, A. Cristina ;

Summers, R. Scott ;

Greenberg, Alan R. ;

Hernandez, Mark T. .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2007, 41 (07) :2491-2497

[17] Deep-ocean gradients in the concentration of dissolved organic carbon [J].

Hansell, DA ;

Carlson, CA .

NATURE, 1998, 395 (6699) :263-266

[18] GLOBAL BIOGEOCHEMICAL CYCLES - PROGRESS AND PROBLEMS [J].

HEDGES, JI .

MARINE CHEMISTRY, 1992, 39 (1-3) :67-93

[19] Optimization of method for detecting and characterizing NOM by HPLC-size exclusion chromatography with UV and on-line DOC detection [J].

Her, N ;

Amy, G ;

Foss, D ;

Cho, J ;

Yoon, Y ;

Kosenka, P .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2002, 36 (05) :1069-1076

[20] Characterization of DOM as a function of MW by fluorescence EEM and HPLC-SEC using UVA, DOC, and fluorescence detection [J].

Her, N ;

Amy, G ;

McKnight, D ;

Sohn, J ;

Yoon, YM .

WATER RESEARCH, 2003, 37 (17) :4295-4303

← 1 2 3 4 →