A uniaxial bioMEMS device for imaging single cell response during quantitative force-displacement measurements

被引：17

作者：

Serrell, David B. ^{[1
,2
]}

Law, Jera ^{[1
]}

Slifka, Andrew J. ^{[1
]}

Mahajan, Roop L. ^{[3
]}

Finch, Dudley S. ^{[3
]}

机构：

[1] Natl Inst Stand & Technol, Boulder, CO 80305 USA

[2] Univ Colorado, Boulder, CO 80309 USA

[3] Virginia Polytech Inst & State Univ, Blacksburg, VA 24061 USA

来源：

BIOMEDICAL MICRODEVICES | 2008年 / 10卷 / 06期

关键词：

actin; bioMEMS; fibroblasts; micro-fabricated; single-cell;

D O I：

10.1007/s10544-008-9202-7

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A microfabricated device has been developed for imaging of a single, adherent cell while quantifying force under an applied displacement. The device works in a fashion similar to that of a displacement-controlled uniaxial tensile machine. The device was calibrated using a tipless atomic force microscope (AFM) cantilever and shows excellent agreement with the calculated spring constant. A step input was applied to a single, adherent fibroblast cell and the viscoelastic response was characterized with a mechanical model. The adherent fibroblast was imaged by use of epifluorescence and phase contrast techniques.

引用

页码：883 / 889

页数：7

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