A methyl viologen-resistant mutant of Arabidopsis, which is allelic to ozone-sensitive rcd1, is tolerant to supplemental ultraviolet-B irradiation

被引:137
作者
Fujibe, T
Saji, H
Arakawa, K
Yabe, N
Takeuchi, Y
Yamamoto, KT
机构
[1] Hokkaido Univ, Grad Sch Environm Earth Sci, Div Biol Sci, Sapporo, Hokkaido 0600810, Japan
[2] Natl Inst Environm Studies, Div Environm Biol, Tsukuba, Ibaraki 3050053, Japan
[3] Hokkaido Univ, Low Temp Inst, Sapporo, Hokkaido 0600819, Japan
[4] Yokohama City Univ, Kihara Inst Biol Res, Grad Sch Integrated Sci, Totsuka Ku, Yokohama, Kanagawa 2440813, Japan
[5] Hokkaido Tokai Univ, Sch Engn, Dept Biosci & Technol, Sapporo, Hokkaido 0058601, Japan
关键词
D O I
10.1104/pp.103.033480
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
To better understand the role of active oxygen species (AOS) in acquired resistance to increased levels of ultraviolet (UV)-B irradiation in plants, we isolated an Arabidopsis mutant that is resistant to methyl viologen, and its sensitivity to UV-B was investigated. A complementation test revealed that the obtained mutant was allelic to the ozone-sensitive radical-induced. cell death1-1 (rcd1-1). Therefore, this mutant was named rcd1-2. rcd1-2 was recessive and nearly 4-fold more resistant to methyl viologen than wild type. It exhibited a higher tolerance to short-term UV-B supplementation treatments than the wild type: UV-B-induced formation of cyclobutane pyrimidine dimers was reduced by one-half after 24 h of exposure; the decrease in quantum yield of photosystem II was also diminished by 40% after 12 h of treatment. Furthermore, rcd1-2 was tolerant to freezing. Steady-state mRNA levels of plastidic Cu/Zn superoxide dismutase and stromal ascorbate peroxidase were higher in rcd1-2 than in wild type, and the mRNA level of the latter enzyme was enhanced by UV-13 exposure more effectively in rcd1-2. UV-B-absorbing compounds were more accumulated in rcd1-2 than in wild type after UV-B exposure for 24 h. These findings suggest that rcd1-2 methyl viologen resistance is due to the enhanced activities of the AOS-scavenging enzymes in chloroplasts and that the acquired tolerance to the short-term UV-B exposure results from a higher accumulation of sunscreen pigments. rcd1 appears to be a mutant that constitutively shows stress responses, leading to accumulation of more pigments and AOS-scavenging enzymes without any stresses.
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收藏
页码:275 / 285
页数:11
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