Quantitative Interrelation between Atractylenolide I, II, and III in Atractylodes japonica Koidzumi Rhizomes, and Evaluation of Their Oxidative Transformation Using a Biomimetic Kinetic Model

被引：29

作者：

Kim, Jung-Hoon ^{[1
]}

Lee, Yuvin ^{[2
]}

Lee, Guemsan ^{[3
]}

Doh, Eui-Jeong ^{[3
,4
]}

Hong, Seungwoo ^{[2
]}

机构：

[1] Pusan Natl Univ, Sch Korean Med, Div Pharmacol, Yangsan 50612, South Korea

[2] Sookmyung Womens Univ, Res Inst Nat Sci, Dept Chem, Seoul 04310, South Korea

[3] Wonkwang Univ, Dept Herbol, Coll Korean Med, Iksan 54538, South Korea

[4] Wonkwang Univ, Res Ctr Tradit Korean Med, Iksan 54538, South Korea

来源：

ACS OMEGA | 2018年 / 3卷 / 11期

基金：

新加坡国家研究基金会;

关键词：

TANDEM MASS-SPECTROMETRY; ESSENTIAL OIL COMPONENTS; LIQUID-CHROMATOGRAPHY; CORRELATION-COEFFICIENT; RAT PLASMA; QUANTIFICATION; IDENTIFICATION; LANCEA; MACROCEPHALA; EXTRACT;

D O I：

10.1021/acsomega.8b02005

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Analytical methods based on ultraperformance liquid chromatography/ion-trap mass spectrometry (UPLC/ion-trap MS) were developed for quantification of atractylenolide I, II, and III in the methanol extract of Atractylodes japonica rhizomes with a C-18 column in an acidified water/acetonitrile gradient eluent in an LC system, and ion-trap MS coupled with electrospray ionization was employed under positive-ion mode. The three atractylenolides were quantified in all A. japonica samples, and the content of atractylenolide I, II, and III showed a significant correlation to each other. Such high correlation was explained by the mechanistic insights into the biosynthetic pathway of atractylenoide III and I from atractylenoide II by using the biomimetic cytochrome P450 model, [Fe(tmp)] (CF3 SO3) (tmp = meso-tetramesitylporphyrin). Atractylenolides could be transformed by oxidation via the oxidative enzyme in the A. japonica plant. The present study first reports the first oxidative transformation of atractylenolides using the heme iron model complex.

引用

页码：14833 / 14840

页数：8

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