Direct sequencing of long polymerase chain reaction fragments

被引：15

作者：

Iannelli, F ^{[1
]}

Giunti, L ^{[1
]}

Pozzi, G ^{[1
]}

机构：

[1] Univ Siena, Dipartimento Biol Mol, Sez Microbiol, I-53100 Siena, Italy

来源：

MOLECULAR BIOTECHNOLOGY | 1998年 / 10卷 / 02期

关键词：

primer walking; DNA purification; automated DNA sequencing; PCR;

D O I：

10.1007/BF02760864

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct sequencing of polymerase chain reaction (PCR)-generated templates is a commonly used technique in molecular biology laboratories. We describe an improved method for direct sequencing of PCR fragments longer than 20 kb obtained with a commercial mixture of Tag and Pwo DNA polymerases. The sequencing protocol was optimized for an automated infrared DNA sequencer, consistently yielding long reads (500-600 bases).

引用

页码：183 / 185

页数：3

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