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Synthesis of 14C-radiolabeled oligonucleotides with a novel phosphoramidite reagent

被引：4
作者：
Guzaev, A
Boyode, B
Balow, G
Tivel, KL
Manoharan, M
机构：
[1] ISIS Pharmaceut, Dept Med Chem, Carlsbad, CA 92008 USA
[2] Moravek Biochem Inc, Brea, CA 92821 USA
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1998年 / 8卷 / 09期
关键词：
D O I：
10.1016/S0960-894X(98)00028-6
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
A novel solid-phase synthesis of 5'-radiolabeled oligonucleotides is described. The labeling reaction is carried out by the phosphoramidite method with the aid of [4,6-di-C-14]-5'-dimethoxytritylthymidine building block 1. The feasibility of the method is demonstrated by preparation of 3'-phosphorylated dodecathymidylate phosphorothioate containing radiolabeled nucleoside at the 5'-terminus. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：1123 / 1126
页数：4
相关论文
共 15 条
[1]  
Cook P.D., 1993, ANTISENSE RES APPL, P149
[2]  
COOK PD, 1991, ANTI-CANCER DRUG DES, V6, P585
[3]  
COSSUM PA, 1993, J PHARMACOL EXP THER, V267, P1181
[4]  
Crooke ST, 1996, J PHARMACOL EXP THER, V277, P923
[5]   TRITIUM LABELING OF ANTISENSE OLIGONUCLEOTIDES BY EXCHANGE WITH TRITIATED-WATER [J].
GRAHAM, MJ ;
FREIER, SM ;
CROOKE, RM ;
ECKER, DJ ;
MASLOVA, RN ;
LESNIK, EA .
NUCLEIC ACIDS RESEARCH, 1993, 21 (16) :3737-3743
[6]   A novel solid support for synthesis of 3'-phosphorylated chimeric oligonucleotides containing internucleosidic methyl phosphotriester and methylphosphonate linkages [J].
Guzaev, A ;
Lonnberg, H .
TETRAHEDRON LETTERS, 1997, 38 (22) :3989-3992
[7]   THE AUTOMATED SYNTHESIS OF SULFUR-CONTAINING OLIGODEOXYRIBONUCLEOTIDES USING 3H-1,2-BENZODITHIOL-3-ONE 1,1-DIOXIDE AS A SULFUR-TRANSFER REAGENT [J].
IYER, RP ;
PHILLIPS, LR ;
EGAN, W ;
REGAN, JB ;
BEAUCAGE, SL .
JOURNAL OF ORGANIC CHEMISTRY, 1990, 55 (15) :4693-4699
[8]   SYNTHESIS OF [S-35] 3H-1,2-BENZODITHIOLE-3-ONE-1,1-DIOXIDE - APPLICATION IN THE PREPARATION OF SITE-SPECIFICALLY S-35 LABELED OLIGONUCLEOTIDES [J].
IYER, RP ;
TAN, WT ;
YU, D ;
AGRAWAL, S .
TETRAHEDRON LETTERS, 1994, 35 (51) :9521-9524
[9]  
MAHLER HR, 1966, BIOL CHEM, P732
[10]  
MANOHARAN M, 1993, ANTISENSE RES APPL, P303
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