Quantitative mass spectrometry identifies insulin signaling targets in C-elegans

被引：245

作者：

Dong, Meng-Qiu

Venable, John D.

Au, Nora

Xu, Tao

Park, Sung Kyu

Cociorva, Daniel

Johnson, Jeffrey R.

Dillin, Andrew

Yates, John R., III ^{[1
]}

机构：

[1] Scripps Res Inst, La Jolla, CA 92037 USA

[2] Salk Inst Biol Studies, La Jolla, CA 92037 USA

[3] Univ Calif San Diego, La Jolla, CA 92093 USA

来源：

SCIENCE | 2007年 / 317卷 / 5838期

关键词：

D O I：

10.1126/science.1139952

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DAF-2, an insulin receptor-like protein, regulates metabolism, development, and aging in Caenorhabditis elegans. In a quantitative proteomic study, we identified 86 proteins that were more or less abundant in long-lived daf-2 mutant worms than in wild-type worms. Genetic studies on a subset of these proteins indicated that they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage and aging. In particular, we discovered a compensatory mechanism activated in response to reduced DAF-2 signaling, which involves the protein phosphatase calcineurin.

引用

页码：660 / 663

页数：4