Size-exclusion chromatography on soft packing material under axial compression

The influence of soft gel axial compression on the packing structure and the associated chromatographic parameters such as dispersion of chromatographic zones, retention volume and peak resolution have been considered for size-exclusion chromatography. Continuous time gel compression is accomplished by the use of a special column with controlled external pressure applied to the packing. The experimentally obtained improvement in resolution combined with the decrease in separation time has been proved.