Effects of staurosporine on exocytosis and endocytosis at frog motor nerve terminals

被引:27
作者
Becherer, U [1 ]
Guatimosim, C [1 ]
Betz, WJ [1 ]
机构
[1] Univ Colorado, Sch Med, Dept Physiol & Biophys, Denver, CO 80262 USA
关键词
staurosporine; exocytosis; endocytosis; synaptic vesicles; frog neuromuscular junction; FM1-43;
D O I
10.1523/JNEUROSCI.21-03-00782.2001
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Observations of the dynamic staining and destaining of FM1-43 in frog motor nerve terminals (Henkel and Betz, 1995) suggested that staurosporine might shorten the interval between exocytosis and endocytosis, inducing a "kiss and run" mode of exocytosis and endocytosis. We tested this hypothesis by using FM1-43 imaging (to measure the time course of FM1-43 endocytosis), intracellular recording of evoked synaptic potentials (to measure acetylcholine release), and electron microscopy (to examine synaptic vesicle distribution). Staurosporine reduced FM1-43 uptake during but not after a tetanus, increased the speed of end plate potential (EPP) amplitude rundown, and greatly slowed the recovery from synaptic depression. Ultrastructural observations showed pronounced vesicle depletion near active zones after tetanic stimulation in staurosporine-treated preparations. These results suggest that staurosporine acted primarily to impair mobilization of synaptic vesicles during tetanic stimulation.
引用
收藏
页码:782 / 787
页数:6
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