Chemical synthesis of 23 kDa glycoprotein by repetitive segment condensation: A synthesis of MUC2 basal motif carrying multiple O-GaINAc moieties

被引：64

作者：

Hojo, H

Matsumoto, Y

Nakahara, Y

Ito, E

Suzuki, Y

Suzuki, M

Suzuki, A

Nakahara, Y

机构：

[1] Tokai Univ, Inst Glycotechnol, Dept Appl Biochem, Hiratsuka, Kanagawa 2591292, Japan

[2] RIKEN, Frontier Res Syst, Sphingolipid Express Lab, Wako, Saitama 3510198, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 39期

关键词：

D O I：

10.1021/ja053711b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Peptide thioester corresponding to a MUC2 tandem repeat unit, which retains seven GaINAc moieties, was prepared by the Fmoc method followed by the low TfOH treatment to remove benzyl groups at the carbohydrate portions. The glycosylated peptide thioester was then consecutively joined by the activation of a thioester group by silver ions to obtain a MUC2 tandem repeat model composed of 141 amino acids with 42 GaINAc moieties.

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页码：13720 / 13725

页数：6

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