New insight into stimulus-induced plasticity of the olfactory epithelium in Mus musculus by quantitative proteomics

被引：14

作者：

Barbour, Jon ^{[1
]}

Neuhaus, Eva M. ^{[2
]}

Piechura, Heike ^{[1
]}

Stoepel, Nadine ^{[1
]}

Mashukova, Anastasia ^{[2
]}

Brunert, Daniela ^{[2
]}

Sitek, Barbara ^{[1
]}

Stuehler, Kai ^{[1
]}

Meyer, Helmut E. ^{[1
]}

Hatt, Hanns ^{[2
]}

Warscheid, Bettina ^{[1
]}

机构：

[1] Ruhr Univ Bochum, Med Proteom Ctr, D-44780 Bochum, Germany

[2] Ruhr Univ Bochum, Dept Cell Physiol, D-44780 Bochum, Germany

来源：

JOURNAL OF PROTEOME RESEARCH | 2008年 / 7卷 / 04期

关键词：

olfactory epithelium; 2-D DIGE; octanal; electro-olfactogram; odorant-binding proteins; desensitization;

D O I：

10.1021/pr7005796

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The olfactory system is exposed to a plethora of chemical compounds throughout an organism's lifespan. Anticipation of stimuli and construction of appropriate neural filters present a significant challenge. This may be addressed via modulation of the protein composition of the sensory epithelium in response to environmental conditions. To reveal the mechanisms governing these changes, we employed a comprehensive quantitative proteomics strategy. Two groups of juvenile mice were treated with either pulsed or continuous application of octanal. After 20 days of treatment, we performed a behavioral study and conducted electrophysiological recordings from the olfactory epithelium (OE). Both treated groups demonstrated peripheral desensitization to octanal; however, only the 'continuous' group exhibited habituation. To obtain novel insight into the molecular mechanisms underpinning the peripheral desensitization to octanal, the OE proteomes of octanal-treated mice versus control were quantitatively analyzed using two-dimensional difference gel electrophoresis. We identified several significantly regulated proteins that were functionally classified as calcium-binding proteins, cytoskeletal proteins, and lipocalins. The calcium-binding proteins and cytoskeletal proteins were up-regulated in the 'pulsed' group, whereas in the 'continuous' group, four lipocalins were significantly down-regulated. Uniquely, the lipocalin odorant-binding protein la was drastically down-regulated in both groups. The identified proteins reflect changes throughout the entire OE, corresponding to changes in neuronal, non-neuronal, and pericellular processes. We report the regulation of several promising candidates for the investigation of odorant-induced changes of the OE. Among these proteins are different lipocalins, which seem to play a crucial role in the regulation of the sensitivity of the olfactory system.

引用

页码：1594 / 1605

页数：12