cDNA sequence and expression of a cold-responsive gene in Citrus unshiu

被引:39
作者
Hara, M
Wakasugi, Y
Ikoma, Y
Yano, M
Ogawa, K
Kuboi, T
机构
[1] Shizuoka Univ, Fac Agr, Dept Environm Sci Human Life, Shizuoka 4228529, Japan
[2] Natl Inst Fruit Tree Sci, Dept Citriculture, Shizuoka 4240204, Japan
关键词
Citrus unshiu; cold stress; dehydrin family; cDNA cloning;
D O I
10.1271/bbb.63.433
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A cDNA clone encoding a protein (CuCOR19), the sequence of which is similar to Poncirus COR19, of the dehydrin family was isolated from the epicarp of Citrus unshiu. The molecular mass of the predicted protein was 18,980 daltons. CuCOR19 was highly hydrophilic and contained three repeating elements including Lys-rich motifs. The gene expression in leaves increased by cold stress.
引用
收藏
页码:433 / 437
页数:5
相关论文
共 35 条
[1]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[2]   AN UNUSUAL GROUP-2 LEA GENE FAMILY IN CITRUS RESPONSIVE TO LOW-TEMPERATURE [J].
CAI, QY ;
MOORE, GA ;
GUY, CL .
PLANT MOLECULAR BIOLOGY, 1995, 29 (01) :11-23
[3]   NUCLEAR-LOCALIZATION OF AGROBACTERIUM VIRE2 PROTEIN IN PLANT-CELLS [J].
CITOVSKY, V ;
ZUPAN, J ;
WARNICK, D ;
ZAMBRYSKI, P .
SCIENCE, 1992, 256 (5065) :1802-1805
[4]   Dehydrins: A commonality in the response of plants to dehydration and low temperature [J].
Close, TJ .
PHYSIOLOGIA PLANTARUM, 1997, 100 (02) :291-296
[6]  
Close TJ, 1993, CUR TOP PL, V10, P104
[7]   NUCLEAR TARGETING SEQUENCES - A CONSENSUS [J].
DINGWALL, C ;
LASKEY, RA .
TRENDS IN BIOCHEMICAL SCIENCES, 1991, 16 (12) :478-481
[8]   COMMON AMINO-ACID SEQUENCE DOMAINS AMONG THE LEA PROTEINS OF HIGHER-PLANTS [J].
DURE, L ;
CROUCH, M ;
HARADA, J ;
HO, THD ;
MUNDY, J ;
QUATRANO, R ;
THOMAS, T ;
SUNG, ZR .
PLANT MOLECULAR BIOLOGY, 1989, 12 (05) :475-486
[9]  
Dure L, 1993, CUR TOP PL, V10, P91
[10]   Salt and oxidative stress: similar and specific responses and their relation to salt tolerance in Citrus [J].
GuetaDahan, Y ;
Yaniv, Z ;
Zilinskas, BA ;
BenHayyim, G .
PLANTA, 1997, 203 (04) :460-469