Involvement of protein kinase C in 5-HT-stimulated ciliary activity in Helisoma trivolvis embryos

被引:21
作者
Christopher, KJ [1 ]
Young, KG [1 ]
Chang, JP [1 ]
Goldberg, JI [1 ]
机构
[1] Univ Alberta, Dept Sci Biol, Edmonton, AB T6G 2E9, Canada
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1999年 / 515卷 / 02期
关键词
D O I
10.1111/j.1469-7793.1999.511ac.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. During development, embryos of the pulmonate gastropod, Helisoma trivolvis, undergo a rotation behaviour due to the co-ordinated beating of three bands of ciliated epithelial cells. This behaviour is in part mediated by the neurotransmitter serotonin (5-HT) released from a pair of identified embryonic neurons. Using time-lapse videomicroscopy to measure ciliary beat frequency (CBF) in response to pharmacological manipulations, we determined whether protein kinase C (PKC) is involved in mediating 5-HT-stimulated ciliary beating. 2. Diacylglycerol (DAG) analogues sn-1,2-dioctanoyl glycerol (DiC(8); 100 mu M) and 1-oleoyl-2-acetyl-sn-glycerol (OAG; 100 mu M), partially mimicked the 5-HT-induced increase in CBF. In contrast, application of OAG in the absence of extracellular Ca2+ did not result in an increase in CBF. 3. 5-HT-stimulated CBF was effectively blocked by PKC inhibitors bisindolylmaleimide (10 and 100 nM) and calphostin C (10 nM). In addition, bisindolylmaleimide (100 nM) inhibited DiC(8)-induced increases in CBF. At a higher concentration (200 nM), bisindolylmaleimide did not significantly reduce 5-HT-stimulated cilio-excitation. 4. Two different phorbol esters, phorbol 12-myristate 13-acetate (TPA; 0.1, 10 or 1000 nM) and phorbol 12 beta, 13 alpha-dibenzoate (PDBn; 10 mu M) did not alter basal CBF. TPA (1 mu M) did not alter 5-HT-stimulated CBF. Likewise, the synthetic form of phosphatidylserine, N-(6-phenylhexyl)-5-chloro-1-naphthalenesulphonamide (SC-9; 10 mu M), did not increase CBF, whereas a strong increase in CBF was observed upon exposure to 5-HT. 5. The results suggest that a DAG-dependent, phorbol ester-insensitive isoform of PKC mediates 5-HT-stimulated CBF in ciliated epithelial cells from embryos of Helisoma trivolvis.
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页码:511 / 522
页数:12
相关论文
共 62 条
[51]   RECONSTITUTION OF CILIARY MEMBRANES CONTAINING TUBULIN [J].
STEPHENS, RE .
JOURNAL OF CELL BIOLOGY, 1983, 96 (01) :68-75
[52]   CALCIUM ACTIVATION OF MUSSEL GILL ABFRONTAL CILIA [J].
STOMMEL, EW .
JOURNAL OF COMPARATIVE PHYSIOLOGY, 1984, 155 (04) :457-469
[53]   CALCIUM REGENERATIVE POTENTIALS IN MYTILUS-EDULIS GILL ABFRONTAL CILIATED EPITHELIAL-CELLS [J].
STOMMEL, EW .
JOURNAL OF COMPARATIVE PHYSIOLOGY, 1984, 155 (04) :445-456
[54]   CYCLIC-AMP AND CALCIUM IN THE DIFFERENTIAL CONTROL OF MYTILUS GILL CILIA [J].
STOMMEL, EW ;
STEPHENS, RE .
JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY, 1985, 157 (04) :451-459
[55]   SPECIFIC LOCALIZATION OF SCALLOP GILL EPITHELIAL CALMODULIN IN CILIA [J].
STOMMEL, EW ;
STEPHENS, RE ;
MASURE, HR ;
HEAD, JF .
JOURNAL OF CELL BIOLOGY, 1982, 92 (03) :622-628
[56]   EFFECT OF CAMP ON CILIARY FUNCTION IN RABBIT TRACHEAL EPITHELIAL-CELLS [J].
TAMAOKI, J ;
KONDO, M ;
TAKIZAWA, T .
JOURNAL OF APPLIED PHYSIOLOGY, 1989, 66 (03) :1035-1039
[58]  
TAUSSIG R, 1989, J NEUROSCI, V9, P3218
[59]  
TOULLEC D, 1991, J BIOL CHEM, V266, P15771
[60]  
WATTS SW, 1994, J PHARMACOL EXP THER, V271, P832