Crystallization and preliminary X-ray diffraction analysis of shikimate kinase from Mycobacterium tuberculosis in complex with MgADP

被引:10
作者
Gu, YJ
Reshetnikova, L
Li, Y
Yan, H
Singh, SV
Ji, XH [1 ]
机构
[1] NCI, Macromol Crystallog Lab, Frederick, MD 21702 USA
[2] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA
[3] Univ Pittsburgh, Sch Med, Dept Pharmacol, Pittsburgh, PA 15261 USA
[4] Univ Pittsburgh, Sch Med, Inst Canc, Pittsburgh, PA 15261 USA
来源
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2001年 / 57卷
关键词
D O I
10.1107/S0907444901014032
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Shikimate kinase (SK) from Mycobacterium tuberculosis (Mt) was overexpressed in Escherichia coli, purified and cocrystallized with MgADP in hanging drops using the vapor-diffusion procedure with PEG 4000 and 2-propanol as precipitants at pH 7.5. The crystal of MtSK-MgADP, which diffracted to 2.2 Angstrom resolution, belonged to space group P3(2)21 or P3(1)21, with unit-cell parameters a=b=64.01, c=92.41 Angstrom. There was one MtSK molecule in the asymmetric unit. Molecular-replacement trials with the crystal structure of SK from Erwinia chrysanthemi (PDB code 1shk) and adenylate kinase (PDB code 1ake) as search models were not successful. Heavy-atom derivative screening is in progress.
引用
收藏
页码:1870 / 1871
页数:2
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