Cell and tissue response to nanotextured Ti6Al4V and Zr implants using high-speed femtosecond laser-induced periodic surface structures

被引：52

作者：

Gnilitskyi, Iaroslav ^{[1
,2
,3
]}

Pogorielov, Maksym ^{[4
,5
]}

Viter, Roman ^{[6
]}

Ferraria, Ana Maria ^{[7
,8
,9
]}

Carapeto, Ana Patricia ^{[7
,8
,9
,10
]}

Oleshko, Oleksandr ^{[4
]}

Orazi, Leonardo ^{[1
]}

Mishchenko, Oleg ^{[5
]}

机构：

[1] Univ Modena & Reggio Emilia, Reggio Emilia, Italy

[2] NoviNano Lab LLC, Lvov, Ukraine

[3] Lviv Polytech Natl Univ, Dept Photon, Lvov, Ukraine

[4] Sumy State Univ, Sumy, Ukraine

[5] Osteoplant R&D, Debice, Poland

[6] Univ Latvia, Inst Atom Phys & Spect, Riga, Latvia

[7] Univ Lisbon, Inst Super Tecn, Ctr Quim Fis Mol, Lisbon, Portugal

[8] Univ Lisbon, Inst Super Tecn, IN, Lisbon, Portugal

[9] Univ Lisbon, Inst Super Tecn, IBB, Lisbon, Portugal

[10] Univ Lisbon, BioISI Biosyst & Integrat Sci Inst, Fac Sci, P-1749016 Lisbon, Portugal

来源：

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | 2019年 / 21卷

基金：

欧盟地平线“2020”;

关键词：

Nanotexturing; Cell proliferation; Fluorescence; HR-LIPSS; Femtosecond laser; PLATELET INTERACTIONS; TITANIUM IMPLANT; DENTAL IMPLANTS; OSTEOBLAST; ROUGHNESS; BEHAVIOR;

D O I：

10.1016/j.nano.2019.102036

中图分类号：

TB3 [工程材料学];

学科分类号：

082905 [生物质能源与材料];

摘要：

In this paper, the effect of femtosecond laser nanotexturing of surfaces of Ti6Al4V and Zr implants on their biological compatibility is presented and discussed. Highly regular and homogeneous nanostructures with sub-micrometer period were imprinted on implant surfaces. Surfaces were morphologically and chemically investigated by SEM and XPS. HDFa cell lines were used for toxicity and cell viability tests, and subcutaneous implantation was applied to characterize tissue response. HDFa proliferation and in vivo experiments evidenced the strong influence of the surface topography compared to the effect of the surface elemental composition (metal or alloy). The effect of protein adsorption from blood plasma on cell proliferation is also discussed. (C) 2019 Elsevier Inc. All rights reserved.

引用

页数：12

共 42 条

[1]

A systematic review of the incidence of biological and technical complications in implant dentistry reported in prospective longitudinal studies of at least 5 years [J].