Liquid chromatographic-mass spectrometric assay for simultaneous pyrimethamine and sulfadoxine determination in human plasma samples

被引：13

作者：

Sinnaeve, BA

Decaestecker, TN

Risha, PG

Remon, JP

Vervaet, C

Van Bocxlaer, JF

机构：

[1] Univ Ghent, Fac Pharmaceut Sci, Lab Med Biochem & Clin Anal, B-9000 Ghent, Belgium

[2] Univ Ghent, Fac Pharmaceut Sci, Pharmaceut Technol Lab, B-9000 Ghent, Belgium

[3] Muhimbili Univ, Coll Hlth Sci, Fac Pharm, Dept Pharmaceut, Dar Es Salaam, Tanzania

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1076卷 / 1-2期

关键词：

sulfadoxine; pyrimethamine; liquid chromatography; mass spectrometry;

D O I：

10.1016/j.chroma.2005.04.047

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a liquid chromatographic-mass spectrometric assay for the simultaneous determination of sulfadoxine and pyrimetharnine in human plasma samples. Sample clean-up was achieved by adding acetonitrile for protein precipitation. Gradient elution in only 10 min resulted in high throughput capability. Tandem mass spectrometric detection in multiple reaction monitoring was used for quantification. The developed analytical approach was successfully validated and was applied in the pharmacokinetic evaluation of the bioavailability between two sulfadoxine/pyrimethamine formulations available on the Eastern African market, using a cross-over design. (c) 2005 Elsevier B.V. All rights reserved.

引用

收藏

页码：97 / 102

页数：6

相关论文

共 17 条

[1] Efficacies of chloroquine, sulfadoxine-pyrimethamine and quinine in the treatment of uncomplicated, Plasmodium falciparum malaria in eastern Sudan [J].

Adam, I ;

Osman, ME ;

Elghzali, G ;

Ahmed, GI ;

Gustafssons, LL ;

Elbashir, MI .

ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 2004, 98 (07) :661-666

[2] Simultaneous determination of pyrimethamine and sulphadoxine in human plasma by high-performance liquid chromatography after automated liquid-solid extraction [J].

Astier, H ;

Renard, C ;

Cheminel, V ;

Soares, O ;

Mounier, C ;

Peyron, F ;

Chaulet, JF .

JOURNAL OF CHROMATOGRAPHY B, 1997, 698 (1-2) :217-223

[3] SIMULTANEOUS DETERMINATION OF PYRIMETHAMINE AND SULFADOXINE IN HUMAN-PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

BERGQVIST, Y ;

ERIKSSON, M .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1985, 79 (03) :297-301

[4] REVERSED-PHASE LIQUID-CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF THE ANTIMALARIAL-DRUGS SULFADOXINE, PYRIMETHAMINE, MEFLOQUINE AND ITS MAJOR CARBOXYLIC METABOLITE IN PLASMA [J].

BERGQVIST, Y ;

ECKERBOM, S ;

LARSSON, H ;

MALEKZADEH, M .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1991, 571 (1-2) :169-177

[5] CONTRIBUTION ON DOSAGE OF 4 ANTI-MALARIAL AGENTS, CHLOROQUINE, QUININE, PYRIMETHAMINE AND SULFADOXINE, ALONE AND COMBINED IN BIOLOGICAL MILIEUX .2. GAS-PHASE CHROMATOGRAPHY [J].

BONINI, M ;

MOKOFIO, F ;

BARAZI, S .

JOURNAL OF CHROMATOGRAPHY, 1981, 224 (02) :332-337

[6]

Burlingame AL, 1998, ANAL CHEM, V70, p647R

[7] QUANTIFICATION OF ANTIMALARIAL-DRUGS .1. SIMULTANEOUS MEASUREMENT OF SULFADOXINE, N4-ACETYLSULFADOXINE AND PYRIMETHAMINE IN HUMAN-PLASMA [J].

EDSTEIN, M .

JOURNAL OF CHROMATOGRAPHY, 1984, 305 (02) :502-507

[8] PHARMACOKINETICS OF SULFADOXINE AND PYRIMETHAMINE AFTER FANSIDAR ADMINISTRATION IN MAN [J].

EDSTEIN, MD .

CHEMOTHERAPY, 1987, 33 (04) :229-233

[9] High-performance liquid chromatography determination of pyrimethamine, dapsone, monoacetyldapsone, sulfadoxine, and N-acetyl-sulfadoxine after rapid solid-phase extraction [J].

Eljaschewitsch, J ;

Padberg, J ;

Schurmann, D ;

Ruf, B .

THERAPEUTIC DRUG MONITORING, 1996, 18 (05) :592-597

[10] High-performance liquid chromatographic assay for the simultaneous determination of sulfadoxine and pyrimethamine from whole blood dried onto filter paper [J].

Green, MD ;

Mount, DL ;

Nettey, H .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 767 (01) :159-162