In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography

被引：87

作者：

Vinegoni, Claudio ^{[1
,2
,3
,4
]}

Pitsouli, Chrysoula ^{[5
,6
]}

Razansky, Daniel ^{[1
,2
,7
,8
]}

Perrimon, Norbert ^{[5
,6
]}

Ntziachristos, Vasilis ^{[1
,2
,7
,8
]}

机构：

[1] Massachusetts Gen Hosp, Ctr Mol Imaging Res, Charlestown, MA 02129 USA

[2] Harvard Univ, Sch Med, Charlestown, MA 02129 USA

[3] Massachusetts Gen Hosp, Ctr Syst Biol, Boston, MA 02114 USA

[4] Harvard Univ, Sch Med, Richard B Simches Res Ctr, Boston, MA 02129 USA

[5] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

[6] Howard Hughes Med Inst, Boston, MA 02129 USA

[7] Tech Univ Munich, Inst Biol & Med Imaging, D-85764 Neuherberg, Germany

[8] Helmholtz Ctr Munich, D-85764 Neuherberg, Germany

来源：

NATURE METHODS | 2008年 / 5卷 / 01期

关键词：

D O I：

10.1038/NMETH1149

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in opaque tissues. We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time.

引用

页码：45 / 47

页数：3

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