Biosynthetic incorporation of fluorohistidine into proteins in E-coli:: A new probe of macromolecular structure

被引：32

作者：

Eichler, JF

Cramer, JC

Kirk, KL

Bann, JG ^{[1
]}

机构：

[1] Wichita State Univ, Dept Chem, Wichita, KS 67226 USA

[2] NIDDKD, Bioorgan Chem Lab, Bethesda, MD 20892 USA

来源：

CHEMBIOCHEM | 2005年 / 6卷 / 12期

关键词：

amino acids; biosynthesis; fluorine; histidine; protein modification;

D O I：

10.1002/cbic.200500249

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Chemical Equation Presented) Fluorine, the other hydrogen: Fluorinated forms of histidine, namely 2- and 4-fluorohistidines (see scheme), have dramatically reduced side-chain pKa's compared to the native amino acid. Biosynthetic incorporation of these analogues into bacterially expressed proteins should, therefore, broaden studies that are aimed at understanding pH-dependent processes. In this study we apply this technique to the production of fluorinated PapD chaperone. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

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收藏

页码：2170 / 2173

页数：4

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