Vectors that facilitate the replacement of transcriptional lacZ fusions in Streptococcus mutans and Bacillus subtilis with fusions to gfp or gusA

被引：8

作者：

Chary, VK ^{[1
]}

Busuioc, M ^{[1
]}

Renye, JA ^{[1
]}

Piggot, PJ ^{[1
]}

机构：

[1] Temple Univ, Sch Med, Dept Microbiol & Immunol, Philadelphia, PA 19140 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 247卷 / 02期

关键词：

Streptococcus mutans; bacillus subtilis; replacement vector;

D O I：

10.1016/j.femsle.2005.05.001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Plasmid vectors have been constructed for Streptococcus mutans and Bacillus subtilis that make possible rapid replacement of the widely used reporter gene lacZ (encoding P-galactosidase) with either gfp (encoding green fluorescent protein) or gusA (encoding beta-glucuronidase). The lacZ gfp replacement vectors greatly facilitate the analysis of the spatial location of gene expression in biofilms of S. mutans and in sporulating B. subtilis. The lacZ -> gusA replacement vectors facilitate the comparison of two promoters within the same organism. A vector is also described that enables gusA to be replaced with gfp in B. subtilis. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：171 / 176

页数：6

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