Neomycin- and spectinomycin-resistance replacement vectors for Bacillus subtilis

被引：14

作者：

Chary, VK ^{[1
]}

Amaya, EI ^{[1
]}

Piggot, PJ ^{[1
]}

机构：

[1] TEMPLE UNIV,SCH MED,DEPT MICROBIOL & IMMUNOL,PHILADELPHIA,PA 19140

来源：

FEMS MICROBIOLOGY LETTERS | 1997年 / 153卷 / 01期

关键词：

Bacillus; plasmid; neomycin; spectinomycin; replacement vector;

D O I：

10.1016/S0378-1097(97)00248-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A plasmid is described for Bacillus subtilis that facilitates replacement of the widely used neomycin resistance gene (neo) with a spectinomycin resistance (spc(E)) gene. A second plasmid is described that facilitates replacement of spc(S), associated with mini-Tn10 mutagenesis in B, subtilis, with neo. These plasmids can also function as integrative vectors for B. subtilis. They expand the scope of strain construction and gene analysis in B. subtilis.

引用

页码：135 / 139

页数：5

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