Nanofiltration for purification of nucleotide sugars

被引：6

作者：

Dudziak, G ^{[1
]}

Fey, S ^{[1
]}

Hasbach, L ^{[1
]}

Kragl, U ^{[1
]}

机构：

[1] Forschungszentrum Julich, Inst Biotechnol, D-52425 Julich, Germany

来源：

JOURNAL OF CARBOHYDRATE CHEMISTRY | 1999年 / 18卷 / 01期

关键词：

D O I：

10.1080/07328309908543977

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nanofiltration was used in downstream processing for the desalination and concentration of the nucleotide sugars, cytidine-5'-monophospho-beta-N-acetylneuraminic acid (CMP-Neu5Ac) 1 and guanosine-5'-diphospho-alpha-D-mannose (GDP-Man) 2. With appropriate membranes, the retention rate for CMP-Neu5Ac was 0.995 and for GDP-Man 0.983. Both nucleotide sugars were purified on a gram scale with a purity greater than or equal to 95%.

引用

页码：41 / 49

页数：9