Direct MALDI-TOF Mass Spectrometry Assay of Blood Culture Broths for Rapid Identification of Candida Species Causing Bloodstream Infections: an Observational Study in Two Large Microbiology Laboratories

被引：161

作者：

Spanu, Teresa ^{[1
]}

Posteraro, Brunella ^{[1
]}

Fiori, Barbara ^{[1
]}

D'Inzeo, Tiziana ^{[1
]}

Campoli, Serena ^{[1
]}

Ruggeri, Alberto ^{[1
]}

Tumbarello, Mario ^{[2
]}

Canu, Giulia ^{[1
]}

Trecarichi, Enrico Maria ^{[2
]}

Parisi, Gabriella ^{[3
]}

Tronci, Mirella ^{[3
]}

Sanguinetti, Maurizio ^{[1
]}

Fadda, Giovanni ^{[1
]}

机构：

[1] Univ Cattolica Sacro Cuore, Ist Microbiol, Rome, Italy

[2] Univ Cattolica Sacro Cuore, Ist Clin Malattie Infett, Rome, Italy

[3] Azienda Osped San Camillo Forlanini, Microbiol Lab, Rome, Italy

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 01期

关键词：

DESORPTION IONIZATION-TIME; INVASIVE CANDIDIASIS; YEAST; MICROORGANISMS; PREDICTORS; MORTALITY; BACTERIA; THERAPY; BOTTLES;

D O I：

10.1128/JCM.05742-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated the reliability of the Bruker Daltonik's MALDI Biotyper system in species-level identification of yeasts directly from blood culture bottles. Identification results were concordant with those of the conventional culture-based method for 95.9% of Candida albicans (187/195) and 86.5% of non-albicans Candida species (128/148). Results were available in 30 min (median), suggesting that this approach is a reliable, time-saving tool for routine identification of Candida species causing bloodstream infection.

引用

页码：176 / 179

页数：4

共 26 条

[1] Hospital Resource Utilization and Costs of Inappropriate Treatment of Candidemia [J].

Arnold, Heather M. ;

Micek, Scott T. ;

Shorr, Andrew F. ;

Zilberberg, Marya D. ;

Labelle, Andrew J. ;

Kothari, Smita ;

Kollef, Marin H. .

PHARMACOTHERAPY, 2010, 30 (04) :361-368

[2] Improved clinical laboratory identification of human pathogenic yeasts by matrix-assisted laser desorption ionization time-of-flight mass spectrometry [J].

Bader, O. ;

Weig, M. ;

Taverne-Ghadwal, L. ;

Lugert, R. ;

Gross, U. ;

Kuhns, M. .

CLINICAL MICROBIOLOGY AND INFECTION, 2011, 17 (09) :1359-1365

[3] Incidence, risk factors, and predictors of outcome of candidemia. Survey in 2 Italian university hospitals [J].

Bassetti, Matteo ;

Trecarichi, Enrico Maria ;

Righi, Elda ;

Sanguinetti, Maurizio ;

Bisio, Francesca ;

Posteraro, Brunella ;

Soro, Ornella ;

Cauda, Roberto ;

Viscoli, Claudio ;

Tumbarello, Mario .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2007, 58 (03) :325-331

[4] Performance and Cost Analysis of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Routine Identification of Yeast [J].

Dhiman, Neelam ;

Hall, Leslie ;

Wohlfiel, Sherri L. ;

Buckwalter, Seanne P. ;

Wengenack, Nancy L. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (04) :1614-1616

[5] Detection of microorganisms in blood specimens using matrix-assisted laser desorption ionization time-of-flight mass spectrometry: a review [J].

Drancourt, M. .

CLINICAL MICROBIOLOGY AND INFECTION, 2010, 16 (11) :1620-1625

[6] Time to positive culture and identification for Candida blood stream infections [J].

Fernandez, Julianna ;

Erstad, Brian L. ;

Petty, Wanda ;

Nix, David E. .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2009, 64 (04) :402-407

[7] Microorganisms direct identification from blood culture by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [J].

Ferreira, L. ;

Sanchez-Juanes, F. ;

Porras-Guerra, I. ;

Garcia-Garcia, M. I. ;

Garcia-Sanchez, J. E. ;

Gonzalez-Buitrago, J. M. ;

Munoz-Bellido, J. L. .

CLINICAL MICROBIOLOGY AND INFECTION, 2011, 17 (04) :546-551

[8] Real-Time Identification of Bacteria and Candida Species in Positive Blood Culture Broths by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry [J].

Ferroni, Agnes ;

Suarez, Stephanie ;

Beretti, Jean-Luc ;

Dauphin, Brunhilde ;

Bille, Emmanuelle ;

Meyer, Julie ;

Bougnoux, Marie-Elisabeth ;

Alanio, Alexandre ;

Berche, Patrick ;

Nassif, Xavier .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (05) :1542-1548

[9] Time to initiation of fluconazole therapy impacts mortality in patients with candidemia: A multi-institutional study [J].

Garey, Kevin W. ;

Rege, Milind ;

Pai, Manjunath P. ;

Mingo, Dana E. ;

Suda, Katie J. ;

Turpin, Robin S. ;

Bearden, David T. .

CLINICAL INFECTIOUS DISEASES, 2006, 43 (01) :25-31

[10] Identification of Candida albicans and Candida glabrata within 1.5 Hours Directly from Positive Blood Culture Bottles with a Shortened Peptide Nucleic Acid Fluorescence In Situ Hybridization Protocol [J].

Gherna, Michael ;

Merz, William G. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (01) :247-248

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