α-Amylase production in high cell density submerged cultivation of Aspergillus oryzae and A-nidulans

被引：20

作者：

Agger, T ^{[1
]}

Spohr, AB ^{[1
]}

Nielsen, J ^{[1
]}

机构：

[1] Tech Univ Denmark, Dept Biotechnol, Ctr Proc Biotechnol, DK-2800 Lyngby, Denmark

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2001年 / 55卷 / 01期

关键词：

D O I：

10.1007/s002530000456

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of biomass concentration on the formation of Aspergillus oryzae alpha -amylase during submerged cultivation with A. oryzae and recombinant A. nidulans strains has been investigated. It was found that the specific rate of alpha -amylase formation in chemostats decreased significantly with increasing biomass concentration in the range of approx. 2-12 g dry weight kg(-1). When using a recombinant A. nidulans strain in which the gene responsible for carbon catabolite repression of the A. oryzae alpha -amylase gene (creA) was deleted, no significant decrease in the specific rate of alpha -amylase formation was observed. On the basis of the experimental results, it is suggested that the low value of the specific alpha -amylase productivity observed at high biomass concentration is caused by slow mixing of the concentrated feed solution in the viscous fermentation medium.

引用

页码：81 / 84

页数：4

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