G proteins are required for spatial orientation of early cell cleavages in C. elegans embryos

被引:130
作者
Zwaal, RR
Ahringer, J
vanLuenen, HGAM
Rushforth, A
Anderson, P
Plasterk, RHA
机构
[1] UNIV CAMBRIDGE,DEPT GENET,CAMBRIDGE CB2 3EH,ENGLAND
[2] MRC,MOL BIOL LAB,CAMBRIDGE CB2 2QH,ENGLAND
[3] UNIV WISCONSIN,DEPT GENET,MADISON,WI 53706
基金
英国惠康基金;
关键词
D O I
10.1016/S0092-8674(00)80135-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Heterotrimeric G proteins are signal-transducing molecules activated by seven transmembrane domain receptors. In C. elegans, gpb-1 encodes the sole G beta subunit; therefore, its inactivation should affect all heterotrimeric G protein signaling. When maternal but no zygotic gpb-1 protein (GPB-1) is present, development proceeds until the first larval stage, but these larvae show little muscle activity and die soon after hatching. When, however, the maternal contribution of GPB-1 is also reduced, spindle orientations in early cell divisions are randomized. Cell positions in these embryos are consequently abnormal, and the embryos die with the normal number of cells and well-differentiated but abnormally distributed tissues. These results indicate that maternal G proteins are important for orientation of early cell division axes, possibly by coupling a membrane signal to centrosome position.
引用
收藏
页码:619 / 629
页数:11
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