Dengue Virus Tropism in Humanized Mice Recapitulates Human Dengue Fever

被引：64

作者：

Mota, Javier ^{[1
]}

Rico-Hesse, Rebeca ^{[1
]}

机构：

[1] SW Fdn Biomed Res, Dept Virol & Immunol, San Antonio, TX USA

来源：

PLOS ONE | 2011年 / 6卷 / 06期

基金：

美国国家卫生研究院;

关键词：

BLOOD MONONUCLEAR-CELLS; PRINCIPAL TARGET-CELLS; HUMAN-BONE-MARROW; HEMORRHAGIC-FEVER; B-CELLS; IMMUNE-RESPONSES; T-CELLS; INFECTION; DISEASE; PATHOGENESIS;

D O I：

10.1371/journal.pone.0020762

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Animal models of dengue virus disease have been very difficult to develop because of the virus' specificity for infection and replication in certain human cells. We developed a model of dengue fever in immunodeficient mice transplanted with human stem cells from umbilical cord blood. These mice show measurable signs of dengue disease as in humans (fever, viremia, erythema and thrombocytopenia), and after infection with the most virulent strain of dengue serotype 2, humanized mice showed infection in human cells in bone marrow, spleen and blood. Cytokines and chemokines were secreted by these human cells into the mouse bloodstream. We demonstrated that the pathology of dengue virus infection in these mice follows that reported in human patients, making this the first valid and relevant model for studying dengue fever pathogenesis in humans.

引用

页数：10

共 51 条

[1] Differential Susceptibility of Aedes aegypti to Infection by the American and Southeast Asian Genotypes of Dengue Type 2 Virus [J].

Armstrong, Philip ;

Rico-Hesse, Rebeca .

VECTOR-BORNE AND ZOONOTIC DISEASES, 2001, 1 (02) :159-+

[2] Tropism of Dengue Virus in Mice and Humans Defined by Viral Nonstructural Protein 3-Specific Immunostaining [J].

Balsitis, Scott J. ;

Coloma, Josefina ;

Castro, Glenda ;

Alava, Aracely ;

Flores, Diana ;

McKerrow, James H. ;

Beatty, P. Robert ;

Harris, Eva .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2009, 80 (03) :416-424

[3] Dengue fever in humanized NOD/SCID mice [J].

Bente, DA ;

Melkus, MW ;

Garcia, JV ;

Rico-Hesse, R .

JOURNAL OF VIROLOGY, 2005, 79 (21) :13797-13799

[4]

Bente Dennis A, 2006, Drug Discov Today Dis Models, V3, P97, DOI 10.1016/j.ddmod.2006.03.014

[5] HEMATODEPRESSIVE VIRUS DISEASES OF THAILAND [J].

BIERMAN, HR ;

NELSON, ER .

ANNALS OF INTERNAL MEDICINE, 1965, 62 (05) :867-+

[6] Primary human splenic macrophages, but not T or B cells, are the principal target cells for dengue virus infection in vitro [J].

Blackley, Shanley ;

Kou, Zhihua ;

Chen, Huiyuan ;

Quinn, Matthew ;

Rose, Robert C. ;

Schlesinger, Jacob J. ;

Coppage, Myra ;

Jin, Xia .

JOURNAL OF VIROLOGY, 2007, 81 (24) :13325-13334

[7]

BOONPUCKNAVIG S, 1976, AM J PATHOL, V85, P37

[8] Parameters for establishing humanized mouse models to study human immunity: Analysis of human hematopoietic stem cell engraftment in three immunodeficient strains of mice bearing the IL2rγnull mutation [J].

Brehm, Michael A. ;

Cuthbert, Amy ;

Yang, Chaoxing ;

Miller, David M. ;

Dilorio, Philip ;

Laning, Joseph ;

Burzenski, Lisa ;

Gott, Bruce ;

Foreman, Oded ;

Kavirayani, Anoop ;

Herlihy, Mary ;

Rossini, Aldo A. ;

Shultz, Leonard D. ;

Greiner, Dale L. .

CLINICAL IMMUNOLOGY, 2010, 135 (01) :84-98

[9] Report of an NIAID workshop on dengue animal models [J].

Cassetti, M. Cristina ;

Durbin, Anna ;

Harris, Eva ;

Rico-Hesse, Rebeca ;

Roehrig, John ;

Rothman, Alan ;

Whitehead, Stephen ;

Natarajan, Ramya ;

Laughlin, Catherine .

VACCINE, 2010, 28 (26) :4229-4234

[10]

Chaturvedi UC, 2000, FEMS IMMUNOL MED MIC, V28, P183, DOI 10.1111/j.1574-695X.2000.tb01474.x

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