Dehydroepiandrosterone (DHEA) stimulates glucose uptake in rat adipocytes: activation of phospholipase D

被引：10

作者：

Ishizawa, M ^{[1
]}

Ishizuka, T ^{[1
]}

Kajita, K ^{[1
]}

Miura, A ^{[1
]}

Kanoh, Y ^{[1
]}

Kimura, M ^{[1
]}

Yasuda, K ^{[1
]}

机构：

[1] Gifu Univ, Sch Med, Dept Gen Med, Dept Internal Med 3, Gifu 5008705, Japan

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2001年 / 130卷 / 03期

关键词：

dehydroepiandrosterone; adrenal androgen; phospholipase D; glucose uptake; rat adipocytes; diacylglycerol; H-3]phosphatidylethanol; methyl-H-3]choline;

D O I：

10.1016/S1096-4959(01)00444-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We examined the effect of dehydroepiandrosterone (DHEA) on glucose uptake and phospholipase D (PLD) activation in rat adipocytes. DHEA (1 muM) provoked a twofold increase in [H-3]2-deoxyglucose (DG) uptake for 30 min. Incorporation of [H-3]-glycerol into diacylglycerol was increased 150% above basal level for 20 min after stimulation with 1 muM DHEA. DHEA increased PLD activity, measured by the incorporation into [H-3]phosphatidylethanol in [H-3]palmitate labelled rat adipocytes, or by [H-3]choline release in [methyl-H-3]choline labeled rat adipocytes. Our results suggest that DHEA stimulates glucose uptake with activation of PLD in rat adipocytes. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：359 / 364

页数：6

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