Novel targeting strategy for generating mouse models with defects in the retinoid cycle

被引：5

作者：

Driessen, C

Winkens, H

Haeseleer, F

Palczewski, K

Janssen, J

机构：

[1] Univ Nijmegen, Dept Biochem 160, NL-6500 HB Nijmegen, Netherlands

[2] Univ Washington, Dept Chem, Seattle, WA 98195 USA

[3] Univ Washington, Dept Pharmacol, Seattle, WA 98195 USA

[4] Univ Washington, Dept Ophthalmol, Seattle, WA 98195 USA

[5] Univ Nijmegen, Dept Ophthalmol, NL-6500 HB Nijmegen, Netherlands

来源：

VISION RESEARCH | 2003年 / 43卷 / 28期

关键词：

retinoid cycle; retinol dehydrogenase; animal model; retinal pathology;

D O I：

10.1016/S0042-6989(03)00483-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In addition to RDH5, other enzymes capable of oxidizing 11-cis-retinol are present within the retinal pigment epithelium, Muller cells and/or photoreceptors. Candidate proteins have meanwhile been identified. To study the physiological and pathological aspects of these enzymes, mice in which these genes are no longer functional are being generated. A fast-targeting strategy for the disruption of genes was developed. Generation of double and triple knockouts will aid in determining if these retinol dehydrogenases are responsible for the remaining 11-cis-retinol oxidation observed in RDH5 knockout animals. (C) 2003 Elsevier Ltd. All rights reserved.

引用

收藏

页码：3075 / 3079

页数：5

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