Potentiation by platelet-derived growth factor-BB of FGF-2-stimulated VEGF release in osteoblasts

被引：13

作者：

Tokuda, Haruhiko ^{[1
,2
]}

Takai, Shinji ^{[2
]}

Hanai, Yoshiteru ^{[1
,2
]}

Harada, Atsushi ^{[3
]}

Matsushima-Nishiwaki, Rie ^{[2
]}

Kato, Hisaaki ^{[2
,4
]}

Ogura, Shinji ^{[4
]}

Kozawa, Osamu ^{[2
]}

机构：

[1] Natl Hosp Geriatr Med, Dept Clin Lab, Natl Ctr Geriatr & Gerontol, Aichi 4748511, Japan

[2] Gifu Univ, Dept Pharmacol, Grad Sch Med, Gifu, Japan

[3] Natl Hosp Geriatr Med, Dept Funct Restorat, Natl Ctr Geriatr & Gerontol, Aichi, Japan

[4] Gifu Univ, Dept Emergency & Disaster Med, Grad Sch Med, Gifu, Japan

来源：

JOURNAL OF BONE AND MINERAL METABOLISM | 2008年 / 26卷 / 04期

关键词：

FGF; PDGF; MAP kinase; VEGF; osteoblast;

D O I：

10.1007/s00774-007-0829-x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We previously reported that basic fibroblast growth factor (FGF-2) stimulates the release of vascular endothelial growth factor (VEGF) via p44/p42 mitogenactivated protein (MAP) kinase and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) in osteoblastlike MC3T3-E1 cells. In the present study, we investigated the effect of platelet-derived growth factor-BB (PDGF-BB) on FGF-2-induced VEGF release in MC3T3-E1 cells. PDGF-BB significantly enhanced the FGF-2-stimulated VEGF release. The amplifying effect of PDGF-BB was dose dependent in the range between 0.1 and 30 ng/ml. AG1295, a selective inhibitor of PDGF receptor kinase, which reduced the autophosphorylation of PDGF receptor-(R), suppressed the enhancement by PDGF-BB without affecting the FGF-2 effect. PDGF-BB failed to strengthen the FGF-2-induced phosphorylation of p44/p42 MAP kinase or SAPK/JNK. The amplification by PDGF-BB of FGF-2-stimulated VEGF release was reduced by PD98059, a specific inhibitor of MEK, or SP600125, a specific inhibitor of SAPK/JNK. These results strongly suggest that PDGF-BB potentiates FGF-2-stimulated VEGF release at a point downstream from p44/p42 MAP kinase and SAPK/JNK in osteoblasts.

引用

页码：335 / 341

页数：7

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