Expression of platelet-activating factor receptor transcript-1 but not transcript-2 by human bone marrow cells

被引：12

作者：

Desplat, V ^{[1
]}

Besse, A ^{[1
]}

Faucher, JL ^{[1
]}

Praloran, V ^{[1
]}

Denizot, Y ^{[1
]}

机构：

[1] Fac Med Limoges, Lab Hematol Expt, F-87025 Limoges, France

来源：

STEM CELLS | 1999年 / 17卷 / 02期

关键词：

bone marrow; CD34(+) progenitors; platelet-activating factor receptor; stromal cells; RT-PCR;

D O I：

10.1002/stem.170121

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The presence of platelet-activating factor receptor (PAF-R) transcripts 1 and 2 was investigated in human bone marrow cells by a reverse transcriptase polymerase chain reaction (RT-PCR) procedure mhich detected their simultaneous presence. RT-PCR experiments reveal PAF-R transcript 1 (but not 2) in freshly isolated mononuclear marrow cells, CD34(+) hematopoietic stem/progenitor cells and cultured marrow stromal cells. For these experiments, the 5637 human bladder carcinoma cell line is used as a positive control for the presence of PAF-R transcripts 1 and 2, Flow cytometry experiments confirm the presence of PAF-R on marrow stromal cells and CD34(+) stem/progenitor cells, In conclusion, the expression of PAF-R transcript 1, which mainly exists in circulating leukocytes, is also found in CD34(+) stem/progenitor cells and cells of the marrow microenvironment, strengthening the potential role of PAF during marrow hematopoiesis.

引用

页码：121 / 124

页数：4

共 15 条

[11] Platelet-activating factor receptor: Gene expression and signal transduction [J].

Izumi, T ;

Shimizu, T .

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1995, 1259 (03) :317-333

[12]

Krause DS, 1996, BLOOD, V87, P1

[13]

MAYANI H, 1992, EUR J HAEMATOL, V49, P225

[14] Platelet-activating factor and antagonists modulate DNA synthesis in human bone marrow stromal cell cultures [J].

Rougier, F ;

Dupuis, F ;

Cornu, E ;

Dulery, C ;

Praloran, V ;

Denizot, Y .

JOURNAL OF LIPID MEDIATORS AND CELL SIGNALLING, 1997, 16 (03) :147-153

[15] Human monocyte-derived and CD34(+) cell-derived dendritic cells express functional receptors for platelet activating factor [J].

Sozzani, S ;

Longoni, D ;

Bonecchi, R ;

Luini, W ;

Bersani, L ;

DAmico, C ;

Borsatti, A ;

Bussolino, F ;

Allavena, P ;

Mantovani, A .

FEBS LETTERS, 1997, 418 (1-2) :98-100

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