DNA detection on transistor arrays following mutation-specific enzymatic amplification

被引:97
作者
Pouthas, F [1 ]
Gentil, C [1 ]
Côte, D [1 ]
Bockelmann, U [1 ]
机构
[1] Ecole Normale Super, Phys Mat Condensee Lab, F-75005 Paris, France
关键词
D O I
10.1063/1.1650907
中图分类号
O59 [应用物理学];
学科分类号
摘要
An integrated array of silicon field-effect transistor structures is used for electronic detection of label-free DNA. Measurements of the dc current-voltage characteristics of the transistors gives us access to reproducible detection of single- and double-stranded DNA, locally adsorbed on the surface of the device. We combine this approach with allele-specific polymerase chain reaction, to test for the 35delG mutation, a frequent mutation related to prelingual nonsyndromic deafness. (C) 2004 American Institute of Physics.
引用
收藏
页码:1594 / 1596
页数:3
相关论文
共 16 条
[2]   The future of biosensors [J].
Bergveld, P .
SENSORS AND ACTUATORS A-PHYSICAL, 1996, 56 (1-2) :65-73
[3]   Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species [J].
Cui, Y ;
Wei, QQ ;
Park, HK ;
Lieber, CM .
SCIENCE, 2001, 293 (5533) :1289-1292
[4]   Prelingual deafness: high prevalence of a 30delG mutation in the connexin 26 gene [J].
Denoyelle, F ;
Weil, D ;
Maw, MA ;
Wilcox, SA ;
Lench, NJ ;
AllenPowell, DR ;
Osborn, AH ;
Dahl, HHM ;
Middleton, A ;
Houseman, MJ ;
Dode, C ;
Marlin, S ;
BoulilaElGgaied, A ;
Grati, M ;
Ayadi, H ;
BenArab, S ;
Bitoun, P ;
LinaGranade, G ;
Godet, J ;
Mustapha, M ;
Loiselet, J ;
ElZir, E ;
Aubois, A ;
Joannard, A ;
Levilliers, J ;
Garabedian, EN ;
Mueller, RF ;
Gardner, RJM ;
Petit, C .
HUMAN MOLECULAR GENETICS, 1997, 6 (12) :2173-2177
[5]   Clinical features of the prevalent form of childhood deafness, DFNB1, due to a connexin-26 gene defect:: implications for genetic counselling [J].
Denoyelle, F ;
Marlin, S ;
Weil, D ;
Moatti, L ;
Chauvin, P ;
Garabédian, EN ;
Petit, C .
LANCET, 1999, 353 (9161) :1298-1303
[6]   A NEURON-SILICON JUNCTION - A RETZIUS CELL OF THE LEECH ON AN INSULATED-GATE FIELD-EFFECT TRANSISTOR [J].
FROMHERZ, P ;
OFFENHAUSSER, A ;
VETTER, T ;
WEIS, J .
SCIENCE, 1991, 252 (5010) :1290-1293
[7]   The use of impedance spectroscopy for the characterization of protein-modified ISFET devices: Application of the method for the analysis of biorecognition processes [J].
Kharitonov, AB ;
Wasserman, J ;
Katz, E ;
Willner, I .
JOURNAL OF PHYSICAL CHEMISTRY B, 2001, 105 (19) :4205-4213
[8]   Extracellular resistance in cell adhesion measured with a transistor probe [J].
Kiessling, V ;
Müller, B ;
Fromherz, P .
LANGMUIR, 2000, 16 (07) :3517-3521
[9]   Chemical amplification: Continuous-flow PCR on a chip [J].
Kopp, MU ;
de Mello, AJ ;
Manz, A .
SCIENCE, 1998, 280 (5366) :1046-1048
[10]  
KWOK PY, 2001, ANNU REV GENOM HUM G, V2, P2001