The transcriptome of the arbuscular mycorrhizal fungus Glomus intraradices (DAOM 197198) reveals functional tradeoffs in an obligate symbiont

被引：260

作者：

Tisserant, E. ^{[1
]}

Kohler, A. ^{[1
]}

Dozolme-Seddas, P. ^{[2
]}

Balestrini, R. ^{[3
,4
]}

Benabdellah, K. ^{[5
]}

Colard, A. ^{[6
,7
]}

Croll, D. ^{[6
,7
]}

Da Silva, C. ^{[8
]}

Gomez, S. K. ^{[9
]}

Koul, R. ^{[10
]}

Ferrol, N. ^{[5
]}

Fiorilli, V. ^{[3
,4
]}

Formey, D. ^{[11
,12
]}

Franken, Ph ^{[13
]}

Helber, N. ^{[14
]}

Hijri, M. ^{[15
]}

Lanfranco, L. ^{[3
,4
]}

Lindquist, E.

Liu, Y. ^{[2
]}

Malbreil, M.

Morin, E. ^{[1
]}

Poulain, J. ^{[8
]}

Shapiro, H. ^{[16
]}

van Tuinen, D. ^{[2
]}

Waschke, A. ^{[13
]}

Azcon-Aguilar, C. ^{[5
]}

Becard, G.

Bonfante, P.

Harrison, M. J. ^{[9
]}

Kuester, H. ^{[17
]}

Lammers, P. ^{[10
]}

Paszkowski, U. ^{[18
]}

Requena, N. ^{[14
]}

Rensing, S. A. ^{[11
,12
,19
,20
]}

Roux, C.

Sanders, I. R.

Shachar-Hill, Y. ^{[21
]}

Tuskan, G. ^{[22
]}

Young, J. P. W. ^{[23
]}

Gianinazzi-Pearson, V. ^{[2
]}

Martin, F. ^{[1
]}

机构：

[1] Univ Henri Poincare, Ctr Nancy, INRA, UMR 1136, F-54280 Champenoux, France

[2] Burgundy Univ Plante Microbe Environm, INRA CMSE, UMR INRA CNRS 1088 5184, F-21065 Dijon, France

[3] Univ Turin, Ist Protez Piante, CNR, Sez Torino, I-10125 Turin, Italy

[4] Univ Turin, Dipartimento Biol Vegetale, I-10125 Turin, Italy

[5] CSIC, Estac Expt Zaidi, Dept Microbiol Suelo & Sistemas Simbiot, E-18008 Granada, Spain

[6] Univ Lausanne, Dept Ecol & Evolut, CH-1015 Lausanne, Switzerland

[7] ETH, CH-8092 Zurich, Switzerland

[8] CEA, IG, F-91057 Evry, France

[9] Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA

[10] New Mexico State Univ, Dept 3MLS, Dept Chem & Biochem, Las Cruces, NM 88003 USA

[11] Univ Toulouse, F-31326 Castanet Tolosan, France

[12] CNRS, UPS, Lab Rech Sci Vegetales, UMR 5546, F-31326 Castanet Tolosan, France

[13] Leibniz Inst Vegetable & Ornamental Crops, Dept Plant Nutr, D-14979 Grossbeeren, Germany

[14] Karlsruhe Inst Technol, Inst Bot, D-76187 Karlsruhe, Germany

[15] Univ Montreal, Inst Rech Biol Vegetale, Dept Sci Biol, Montreal, PQ H1X 2B2, Canada

[16] Joint Genome Inst, Walnut Creek, CA 94598 USA

[17] Leibniz Univ Hannover, Inst Pflanzengenet, D-30419 Hannover, Germany

[18] Univ Lausanne, Dept Biol Mol Vegetale, CH-1015 Lausanne, Switzerland

[19] Univ Freiburg, BIOSS Ctr Biol Signalling Studies, Freiburg Initiat Syst Biol, D-79104 Freiburg, Germany

[20] Univ Freiburg, Fac Biol, D-79104 Freiburg, Germany

[21] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA

[22] Oak Ridge Natl Lab, Oak Ridge, TN 37831 USA

[23] Univ York, Dept Biol, York YO10 5DD, N Yorkshire, England

来源：

NEW PHYTOLOGIST | 2012年 / 193卷 / 03期

基金：

美国国家科学基金会;

关键词：

Glomeromycota; Glomus; meiosis and recombination genes; mycorrhiza; small secreted proteins; symbiosis; transcript profiling; PHOSPHATE TRANSPORTER GENES; AMMONIUM TRANSPORTER; NITROGEN TRANSFER; LACCARIA-BICOLOR; CELL-WALL; PLANT; EXPRESSION; GENOME; EFFECTOR; POLYPHOSPHATE;

D O I：

10.1111/j.1469-8137.2011.03948.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The arbuscular mycorrhizal symbiosis is arguably the most ecologically important eukaryotic symbiosis, yet it is poorly understood at the molecular level. To provide novel insights into the molecular basis of symbiosis-associated traits, we report the first genome-wide analysis of the transcriptome from Glomus intraradices DAOM 197198. We generated a set of 25 906 nonredundant virtual transcripts (NRVTs) transcribed in germinated spores, extraradical mycelium and symbiotic roots using Sanger and 454 sequencing. NRVTs were used to construct an oligoarray for investigating gene expression. We identified transcripts coding for the meiotic recombination machinery, as well as meiosis-specific proteins, suggesting that the lack of a known sexual cycle in G.intraradices is not a result of major deletions of genes essential for sexual reproduction and meiosis. Induced expression of genes encoding membrane transporters and small secreted proteins in intraradical mycelium, together with the lack of expression of hydrolytic enzymes acting on plant cell wall polysaccharides, are all features of G.intraradices that are shared with ectomycorrhizal symbionts and obligate biotrophic pathogens. Our results illuminate the genetic basis of symbiosis-related traits of the most ancient lineage of plant biotrophs, advancing future research on these agriculturally and ecologically important symbionts.

引用

页码：755 / 769

页数：15

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