Fmoc chemistry compatible thio-ligation assembly of proteins

被引：24

作者：

Biancalana, S

Hudson, D

Songster, MF

Thompson, SA

机构：

[1] Biosearch Technol Inc, Novato, CA 94949 USA

[2] Berlex Biosci, Richmond, CA 94804 USA

来源：

LETTERS IN PEPTIDE SCIENCE | 2000年 / 7卷 / 05期

关键词：

chemoselective ligation; Fmoc-methodology; iso-thiouronium salts; Kenner safety catch resin; native chemical ligation; peptide thioesters; peptide isomerisation; safety-catch linkers; solid-phase synthesis; thioester exchange;

D O I：

10.1023/A:1011849912229

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe, and compare, two methods which enable the assembly of proteins from peptide thioester fragments prepared by Fmoc chemistry mediated solid phase synthesis. The first, which utilizes iso-thiouronium salts, allows formation of thiophenyl esters directly from partially protected peptides, either in solution or on resin. The second uses `sulfonamide' based safety-catch resins. Data on yields, generality and potential for side-reactions are provided.

引用

收藏

页码：291 / 297

页数：7

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