共 11 条
[11]
TAM JP, 1999, INNOVATION PERSPECTI, P1
Fmoc chemistry compatible thio-ligation assembly of proteins
被引：24
作者：
Biancalana, S
Hudson, D
Songster, MF
Thompson, SA
机构：
[1] Biosearch Technol Inc, Novato, CA 94949 USA
[2] Berlex Biosci, Richmond, CA 94804 USA
来源：
LETTERS IN PEPTIDE SCIENCE
|
2000年
/
7卷
/
05期
关键词：
chemoselective ligation;
Fmoc-methodology;
iso-thiouronium salts;
Kenner safety catch resin;
native chemical ligation;
peptide thioesters;
peptide isomerisation;
safety-catch linkers;
solid-phase synthesis;
thioester exchange;
D O I：
10.1023/A:1011849912229
中图分类号：
Q5 [生物化学];
Q7 [分子生物学];
学科分类号：
071010 ;
081704 ;
摘要：
We describe, and compare, two methods which enable the assembly of proteins from peptide thioester fragments prepared by Fmoc chemistry mediated solid phase synthesis. The first, which utilizes iso-thiouronium salts, allows formation of thiophenyl esters directly from partially protected peptides, either in solution or on resin. The second uses `sulfonamide' based safety-catch resins. Data on yields, generality and potential for side-reactions are provided.
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页码：291 / 297
页数：7
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