A new small coil-volume CCC instrument for direct interfacing with MS

被引：27

作者：

Janaway, L

Hawes, D

Ignatova, S

Wood, P

Sutherland, IA ^{[1
]}

机构：

[1] Brunel Univ, Brunel Inst Bioengn, Uxbridge UB8 3PH, Middx, England

[2] Russian Acad Sci, Vernadsky Inst Geochem & Analyt Chem, Moscow, Russia

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2003年 / 26卷 / 9-10期

关键词：

small volume CCC machine; J-type coil planet centrifuge; mass spectrometry coupling;

D O I：

10.1081/JLC-120021254

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

One of the major factors restricting the use of CCC as an analytical tool is the speed at which a separation may be conducted. This paper describes the phased development of a new low volume capacity Milli-CCC device, which is as rapid as HPLC, achieving high resolutions in minutes as opposed to hours, with the capability of linking with a mass spectrometer (CCC/MS). The Milli-CCC J-type apparatus has gears enclosed in a lubricated case to minimize noise. Its volume with one coil mounted in a cantilever style is 4.6 mL with 2.5 m of 0.76 nun bore tubing. It can rotate at a maximum speed of 2 100 rpm. Stationary phase retention factor higher than 60% could be obtained with 1500 rpm and 1 mL/min producing separation of compounds with K-D distribution coefficient of 1 in less than 5 min. The connection to MS was straightforward.

引用

页码：1345 / 1354

页数：10