In vitro evaluation of phenolic-enriched maple syrup extracts for inhibition of carbohydrate hydrolyzing enzymes relevant to type 2 diabetes management

被引：82

作者：

Apostolidis, Emmanouil ^{[2
]}

Li, Liya ^{[1
]}

Lee, Chong ^{[2
]}

Seeram, Navindra P. ^{[1
]}

机构：

[1] Univ Rhode Isl, Dept Biomed & Pharmaceut Sci, Bioact Bot Res Lab, Kingston, RI 02881 USA

[2] Univ Rhode Isl, Food Sci & Nutr Res Ctr, Dept Food Sci & Nutr, W Kingston, RI 02892 USA

来源：

JOURNAL OF FUNCTIONAL FOODS | 2011年 / 3卷 / 02期

关键词：

Maple syrup; Phenolics; Type; 2; diabetes; alpha-Glucosidase inhibition; alpha-Amylase inhibition; ANTIOXIDANT ACTIVITY; HEALTH-BENEFITS; POLYPHENOLS; IDENTIFICATION; HYPERGLYCEMIA; CHEMISTRY; SAP;

D O I：

10.1016/j.jff.2011.03.003

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

Phenolic-enriched extracts of Canadian maple syrup, namely ethyl acetate (MS-EtOAc) and butanol (MS-BuOH), were evaluated for their ability to inhibit carbohydrate hydrolyzing enzymes relevant to type 2 diabetes management. Extracts were standardized to phenolic contents by the Folin-Ciocalteau method and assayed for yeast alpha-glucosidase inhibitory activities. On normalization to phenolic content, MS-BuOH exhibited higher inhibitory activity than MS-EtOAc (IC50 = 68.38 and 107.9 mu g phenolics, respectively). The extracts were further assayed for inhibition of porcine alpha-amylase and rat alpha-glucosidase enzymes. MS-BuOH exhibited higher rat alpha-glucosidase and porcine alpha-amylase inhibitory activities (IC50 = 135 and 103 mu g phenolics, respectively) than MS-EtOAC extract (IC50 > 187 mu g phenolics in both assays). These results suggest that maple syrup extracts may have potential for phenolic-mediated type 2 diabetes management, with the MS-BuOH phenolic-enriched fraction having highest bioactivity. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：100 / 106

页数：7

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