A new human nm23 homologue (nm23-H5) specifically expressed in testis germinal cells

被引：101

作者：

Munier, A

Feral, C

Milon, L

Pinon, VPB

Gyapay, G

Capeau, J

Guellaen, G

Lacombe, ML ^{[1
]}

机构：

[1] Fac Med St Antoine, INSERM, U402, Paris, France

[2] Hop Henri Mondor, INSERM, U99, Creteil, France

[3] Genethon, CNRS, URA 1922, Evry, France

来源：

FEBS LETTERS | 1998年 / 434卷 / 03期

关键词：

nucleoside diphosphate kinase; nm23; testis; metastasis; cancer;

D O I：

10.1016/S0014-5793(98)00996-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified a cDNA encoding a 212 amino acid protein (Nm23-H5) with 27-31% identity to the human members of the nm23/nucleoside diphosphate (NDP) kinase gene family. The nm23-H5 gene is located on chromosome 5q23-31 and is transcribed as one main transcript of 1.1 kb which is highly and specifically expressed in testis, in the spermatogonia and early spermatocytes, Nm23-H5 possesses most of the residues conserved among NDP kinases plus an additional COOH-terminus end of 55 amino acids unique to this protein. However, under usual assay conditions, Nm23-H5 expressed in Escherichia coli did not exhibit NDP kinase activity. These results suggest that Nm23-H5 is specifically involved in early stages of spermatogenesis. (C) 1998 Federation of European Biochemical Societies.

引用

页码：289 / 294

页数：6

共 28 条

[1]

Agarwal R P, 1978, Methods Enzymol, V51, P376

[2]

BACKER JM, 1993, ONCOGENE, V8, P497

[3] ANALYSIS OF THE LETHAL INTERACTION BETWEEN THE PRUNE AND KILLER OF PRUNE MUTATIONS OF DROSOPHILA [J].