Identification of a sex-determining region in Nile tilapia (Oreochromis niloticus) using bulked segregant analysis

被引:143
作者
Lee, BY
Penman, DJ
Kocher, TD
机构
[1] Univ New Hampshire, Hubbard Ctr Genome Studies, Durham, NH 03824 USA
[2] Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland
关键词
aquaculture; Oreochromis; sex chromosomes; sex linkage; tilapia;
D O I
10.1046/j.1365-2052.2003.01035.x
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Sex determination in the Nile tilapia (Oreochromis niloticus) is thought to be an XX-XY (male heterogametic) system controlled by a major gene. We searched for DNA markers linked to this major locus using bulked segregant analysis. Ten microsatellite markers belonging to linkage group 8 were found to be linked to phenotypic sex. The putative Y-chromosome alleles correctly predict the sex of 95% of male and female individuals in two families. Our results suggest a major sex-determining locus within a few centimorgans of markers UNH995 and UNH104. A third family from the same population showed no evidence for linkage of this region with phenotypic sex, indicating that additional genetic and/or environmental factors regulate sex determination in some families. These markers have immediate utility for studying the strength of different Y chromosome alleles, and for identifying broodstock carrying one or more copies of the Y haplotype.
引用
收藏
页码:379 / 383
页数:5
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