Identification of a sex-determining region in Nile tilapia (Oreochromis niloticus) using bulked segregant analysis

被引：143

作者：

Lee, BY

Penman, DJ

Kocher, TD

机构：

[1] Univ New Hampshire, Hubbard Ctr Genome Studies, Durham, NH 03824 USA

[2] Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland

来源：

ANIMAL GENETICS | 2003年 / 34卷 / 05期

关键词：

aquaculture; Oreochromis; sex chromosomes; sex linkage; tilapia;

D O I：

10.1046/j.1365-2052.2003.01035.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Sex determination in the Nile tilapia (Oreochromis niloticus) is thought to be an XX-XY (male heterogametic) system controlled by a major gene. We searched for DNA markers linked to this major locus using bulked segregant analysis. Ten microsatellite markers belonging to linkage group 8 were found to be linked to phenotypic sex. The putative Y-chromosome alleles correctly predict the sex of 95% of male and female individuals in two families. Our results suggest a major sex-determining locus within a few centimorgans of markers UNH995 and UNH104. A third family from the same population showed no evidence for linkage of this region with phenotypic sex, indicating that additional genetic and/or environmental factors regulate sex determination in some families. These markers have immediate utility for studying the strength of different Y chromosome alleles, and for identifying broodstock carrying one or more copies of the Y haplotype.

引用

页码：379 / 383

页数：5

共 25 条

[11]

HUSSAIN MG, 1994, GENETICS AND EVOLUTION OF AQUATIC ORGANISMS, P502

[12] SEX DETERMINATION IN TILAPIA-MACROCHIR AND TILAPIA-NILOTICA - EFFECT OF METHYLTESTOSTERONE ADMINISTERED IN FRY FOOD ON SEX DIFFERENTIATION - SEX-RATIO OF OFFSPRING PRODUCED BY SEX-REVERSED MALES [J].