Rapid and Sensitive Loop-Mediated Isothermal Amplification Test for Clostridium difficile Detection Challenges Cytotoxin B Cell Test and Culture as Gold Standard

被引：56

作者：

Noren, Torbjorn ^{[1
,2
]}

Alriksson, Ingegard ^{[1
]}

Andersson, Josefin ^{[1
]}

Akerlund, Thomas ^{[3
]}

Unemo, Magnus ^{[1
]}

机构：

[1] Orebro Univ Hosp, Dept Lab Med, SE-70185 Orebro, Sweden

[2] Orebro Univ Hosp, Dept Infect Dis, SE-70185 Orebro, Sweden

[3] Swedish Inst Infect Dis Control, Dept Bacteriol, Solna, Sweden

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 02期

关键词：

TIME PCR ASSAY; ENZYME-IMMUNOASSAY; TOXIN-B; DIAGNOSIS; ALGORITHM; ANTIGEN;

D O I：

10.1128/JCM.01824-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Compared to the composite gold standard cytotoxin B assay and toxigenic culture, the loop-mediated isothermal amplification (LAMP) test for Clostridium difficile had a sensitivity and specificity of 98%, positive predictive value of 92%, and negative predictive value of >99%. A one-hour turnaround time for the LAMP test provides rapid diagnosis and cost savings.

引用

页码：710 / 711

页数：2

共 23 条

[1] Clinical recognition and diagnosis of Clostridium difficile infection [J].

Bartlett, John G. ;

Gerding, Dale N. .

CLINICAL INFECTIOUS DISEASES, 2008, 46 :S12-S18

[2] LATEX PARTICLE AGGLUTINATION FOR DETECTING AND IDENTIFYING CLOSTRIDIUM-DIFFICILE [J].

BOWMAN, RA ;

ARROW, SA ;

RILEY, TV .

JOURNAL OF CLINICAL PATHOLOGY, 1986, 39 (02) :212-214

[3] European Society of Clinical Microbiology and Infectious Diseases (ESCMID): Data review and recommendations for diagnosing Clostridium difficile-infection (CDI) [J].

Crobach, M. J. T. ;

Dekkers, O. M. ;

Wilcox, M. H. ;

Kuijper, E. J. .

CLINICAL MICROBIOLOGY AND INFECTION, 2009, 15 (12) :1053-1066

[4] Laboratory diagnosis of Clostridium difficile associated diarrhoea:: a plea for culture [J].

Delmée, M ;

Van Broeck, J ;

Simon, A ;

Janssens, M ;

Avesani, V .

JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 54 (02) :187-191

[5] Toxin A-negative, toxin B-positive Clostridium difficile [J].

Drudy, Denise ;

Fanning, Seamus ;

Kyne, Lorraine .

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2007, 11 (01) :5-10

[6] Is a two-step glutamate dehyrogenase antigen-cytotoxicity neutralization assay algorithm superior to the premier toxin a and B enzyme immunoassay for laboratory detection of Clostridium difficile? [J].

Gilligan, Peter H. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (04) :1523-1525

[7] Two-step glutamate dehydrogenase antigen real-time polymerase chain reaction assay for detection of toxigenic Clostridium difficile [J].

Goldenberg, S. D. ;

Cliff, P. R. ;

Smith, S. ;

Milner, M. ;

French, G. L. .

JOURNAL OF HOSPITAL INFECTION, 2010, 74 (01) :48-54

[8] Induction of toxins in Clostridium difficile is associated with dramatic changes of its metabolism [J].

Karlsson, Sture ;

Burman, Lars G. ;

Akerlund, Thomas .

MICROBIOLOGY-SGM, 2008, 154 :3430-3436

[9] Comparison of BD GeneOhm Cdiff Real-Time PCR Assay with a Two-Step Algorithm and a Toxin A/B Enzyme-Linked Immunosorbent Assay for Diagnosis of Toxigenic Clostridium difficile Infection [J].

Kvach, Elizabeth J. ;

Ferguson, David ;

Riska, Paul F. ;

Landry, Marie L. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (01) :109-114

[10] Asymptomatic carriage of Clostridium difficile and serum levels of IgG antibody against toxin A. [J].

Kyne, L ;

Warny, M ;

Qamar, A ;

Kelly, CP .

NEW ENGLAND JOURNAL OF MEDICINE, 2000, 342 (06) :390-397

← 1 2 3 →