学术探索
学术期刊
新闻热点
数据分析
智能评审

An exceptionally selective lead(II)-regulatory protein from Ralstonia metallidurans:: Development of a fluorescent lead(II) probe

被引：149
作者：
Chen, P
Greenberg, B
Taghavi, S
Romano, C
van der Lelie, D
He, CA
机构：
[1] Univ Chicago, Dept Chem, Chicago, IL 60637 USA
[2] Brookhaven Natl Lab, Biol Dept, Upton, NY 11973 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 18期
关键词：
fluorescent probes; lead; PbrR; regulatory proteins; sensors;
D O I：
10.1002/anie.200462443
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Chemical Equation Presented) Lighting up lead: A Pb2+- regulatory protein from Ralstonia metallidurans has been converted into a highly sensitive fluorescent Pb2+ probe (PbR691, turquoise circles in scheme). This fluorescent system reveals that the protein exhibits a surprisingly high binding selectivity towards Pb2+ ions over other metal ions. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：2715 / 2719
页数：5
相关论文
共 46 条
  • [1] [Anonymous], 1997, J FLUORESC
  • [2] ANSARI AZ, 1995, NATURE, V374, P371
  • [3] Recent developments in transition metal ion detection by luminescent chemosensors
    Bargossi, C
    Fiorini, MC
    Montalti, M
    Prodi, L
    Zaccheroni, N
    [J]. COORDINATION CHEMISTRY REVIEWS, 2000, 208 : 17 - 32
  • [4] Design of bioelectronic interfaces by exploiting hinge-bending motions in proteins
    Benson, DE
    Conrad, DW
    de Lorimer, RM
    Trammell, SA
    Hellinga, HW
    [J]. SCIENCE, 2001, 293 (5535) : 1641 - 1644
  • [5] Cloning and functional analysis of the pbr lead resistance determinant of Ralstonia metallidurans CH34
    Borremans, B
    Hobman, JL
    Provoost, A
    Brown, NL
    Van der Lelie, D
    [J]. JOURNAL OF BACTERIOLOGY, 2001, 183 (19) : 5651 - 5658
  • [6] Bis-8-hydroxyquinoline-armed diazatrithia-15-crown-5 and diazatrithia-16-crown-5 ligands: Possible fluorophoric metal ion sensors
    Bronson, RT
    Bradshaw, JS
    Savage, PB
    Fuangswasdi, S
    Lee, SC
    Krakowiak, KE
    Izatt, RM
    [J]. JOURNAL OF ORGANIC CHEMISTRY, 2001, 66 (14) : 4752 - 4758
  • [7] The MerR family of transcriptional regulators
    Brown, NL
    Stoyanov, JV
    Kidd, SP
    Hobman, JL
    [J]. FEMS MICROBIOLOGY REVIEWS, 2003, 27 (2-3) : 145 - 163
  • [8] Fluorescent sensors for Zn2+ based on a fluorescein platform:: Synthesis, properties and intracellular distribution
    Burdette, SC
    Walkup, GK
    Spingler, B
    Tsien, RY
    Lippard, SJ
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (32) : 7831 - 7841
  • [9] Chae MY, 1996, J MOL RECOGNIT, V9, P297, DOI 10.1002/(SICI)1099-1352(199607)9:4<297::AID-JMR335>3.0.CO
  • [10] 2-6
12345