An exceptionally selective lead(II)-regulatory protein from Ralstonia metallidurans:: Development of a fluorescent lead(II) probe

被引：149

作者：

Chen, P

Greenberg, B

Taghavi, S

Romano, C

van der Lelie, D

He, CA

机构：

[1] Univ Chicago, Dept Chem, Chicago, IL 60637 USA

[2] Brookhaven Natl Lab, Biol Dept, Upton, NY 11973 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 18期

关键词：

fluorescent probes; lead; PbrR; regulatory proteins; sensors;

D O I：

10.1002/anie.200462443

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Lighting up lead: A Pb2+- regulatory protein from Ralstonia metallidurans has been converted into a highly sensitive fluorescent Pb2+ probe (PbR691, turquoise circles in scheme). This fluorescent system reveals that the protein exhibits a surprisingly high binding selectivity towards Pb2+ ions over other metal ions. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2715 / 2719

页数：5

共 46 条

[21] Crystal structure of the transcription activator BmrR bound to DNA and a drug
Heldwein, EEZ
Brennan, RG
[J]. NATURE, 2001, 409 (6818) : 378 - 382
[22] Zinc(II)-selective ratiometric fluorescent sensors based on inhibition of excited-state intramolecular proton transfer
Henary, MM
Wu, YG
Fahrni, CJ
[J]. CHEMISTRY-A EUROPEAN JOURNAL, 2004, 10 (12) : 3015 - 3025
[23] 2-Aminopurine electronic structure and fluorescence properties in DNA
Jean, JM
Hall, KB
[J]. BIOCHEMISTRY, 2002, 41 (44) : 13152 - 13161
[24] Cellular uptake of lead is activated by depletion of intracellular calcium stores
Kerper, LE
Hinkle, PM
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (13) : 8346 - 8352
[25] Krämer R, 1998, ANGEW CHEM INT EDIT, V37, P772, DOI 10.1002/(SICI)1521-3773(19980403)37:6<772::AID-ANIE772>3.0.CO
[26] 2-Z
[27] LANDRIGAN PJ, 1994, WESTERN J MED, V161, P153
[28] A highly sensitive and selective catalytic DNA biosensor for lead ions
Li, J
Lu, Y
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (42) : 10466 - 10467
[29] A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles
Liu, JW
Lu, Y
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (22) : 6642 - 6643
[30] Optimization of a Pb2+-directed gold nanoparticle/DNAzyme assembly and its application as a colorimetric biosensor for Pb2+
Liu, JW
Lu, Y
[J]. CHEMISTRY OF MATERIALS, 2004, 16 (17) : 3231 - 3238

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