High-throughput miniature cylindrical ion trap array mass spectrometer

被引:54
作者
Tabert, AM [1 ]
Griep-Raming, J [1 ]
Guymon, AJ [1 ]
Cooks, RG [1 ]
机构
[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
关键词
D O I
10.1021/ac0346858
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A fully multiplexed cylindrical ion trap (CIT) array mass spectrometer with four parallel ion source/mass analyzer/detector channels has been built to allow simultaneous high-throughput analysis of multiple samples. A multi-element external chemical ionization/electron ionization source was coupled to a parallel array of CITs each of equal size (internal radius 2.5 mm), and the signal was recorded using an array of four miniature (2-mm inner diameter) electron multipliers. Using external electron ionization, the spectra of four separate samples were recorded simultaneously in real time using a four-channel preamplifier system and a data acquisition program written using LabVIEW software. These experiments mark the first demonstration of externally generated ions being successfully trapped in a miniature CIT mass analyzer. The instrument currently provides mass/charge range of approximately m/z 50-500. Average peak width is m/z 0.3, corresponding to a resolution of 1000 at m/z 300. The four-channel mass spectrometer is housed in a single vacuum manifold and operated with a single set of control electronics. The modular design of this instrument allows scale-up to many more channels of analysis for future applications in the areas of industrial process monitoring and combinatorial analysis and in the fields of proteomics and metabolomics.
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收藏
页码:5656 / 5664
页数:9
相关论文
共 48 条
[1]   Mass spectrometry in proteomics [J].
Aebersold, R ;
Goodlett, DR .
CHEMICAL REVIEWS, 2001, 101 (02) :269-295
[2]   Cylindrical ion trap array with mass selection by variation in trap dimensions [J].
Badman, ER ;
Cooks, RG .
ANALYTICAL CHEMISTRY, 2000, 72 (20) :5079-5086
[3]   Fourier transform detection in a cylindrical quadrupole ion trap [J].
Badman, ER ;
Wells, JM ;
Bui, HA ;
Cooks, RG .
ANALYTICAL CHEMISTRY, 1998, 70 (17) :3545-3547
[4]   A parallel miniature cylindrical ion trap array [J].
Badman, ER ;
Cooks, RG .
ANALYTICAL CHEMISTRY, 2000, 72 (14) :3291-3297
[5]   A miniature cylindrical quadrupole ion trap: Simulation and experiment [J].
Badman, ER ;
Johnson, RC ;
Plass, WR ;
Cooks, RG .
ANALYTICAL CHEMISTRY, 1998, 70 (23) :4896-4901
[6]  
BADMAN ER, 2001, THESIS PURDUE U W LA
[7]   Trade-offs in miniature quadrupole designs [J].
Boumsellek, S ;
Ferran, RJ .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2001, 12 (06) :633-640
[8]  
Bui HA, 1998, J MASS SPECTROM, V33, P297, DOI 10.1002/(SICI)1096-9888(199804)33:4<297::AID-JMS665>3.3.CO
[9]  
2-M
[10]  
COOKS RG, 2002, PITTSB C AN CHEM APP