Chicken microchromosomes are hypermethylated and can be identified by specific painting probes

被引：25

作者：

Grützner, F

Zend-Ajusch, E

Stout, K

Munsche, S

Niveleau, A

Nanda, I

Schmid, M

Haaf, T

机构：

[1] Max Planck Inst Mol Genet, D-14195 Berlin, Germany

[2] Univ Grenoble 1, Mol & Struct Virol Unit, La Tronche, France

[3] Univ Wurzburg, Dept Human Genet, D-8700 Wurzburg, Germany

来源：

CYTOGENETICS AND CELL GENETICS | 2001年 / 93卷 / 3-4期

关键词：

D O I：

10.1159/000056996

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Microdissection of single chicken microchromosomes (MICs) followed by degenerate oligonucleotide-primed (DOP) PCR allows the rapid generation of MIC-specific DNA libraries. Since some libraries derived from a single (or a few) chromosome(s) label the entire MIC fraction, the majority of chicken MICs share repetitive DNA sequences that are not found on the macrochromosomes. In evolutionarily distant bird species, MICs are invariably hypermethylated. Methylcytosine staining provides additional in situ evidence for the high gene content of MICs and strong compartmentalization of avian genomes. Copyright (C) 2001 S. Karger Ag, Basel.

引用

页码：265 / 269

页数：5

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