Appearance of glucocorticoid receptors in the inner ear of the mouse during development

被引:35
作者
Erichsen, S
BaggerSjoback, D
Curtis, L
Zuo, J
Rarey, K
Hultcrantz, M
机构
[1] KAROLINSKA HOSP, DEPT OTORHINOLARYNGOL, S-17176 STOCKHOLM, SWEDEN
[2] UNIV FLORIDA, DEPT ANAT & CELL BIOL, GAINESVILLE, FL USA
[3] UNIV FLORIDA, DEPT OTOLARYNGOL, GAINESVILLE, FL USA
关键词
embryology; immunohistochemistry; glucocorticoid receptors; mouse; cochlea; vestibular labyrinth;
D O I
10.3109/00016489609137913
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 ;
摘要
CBA mice were sacrificed at different ages of development: at embryonic day 13 (E13), E14, E16, E19, E20 and postnatal day 1 (P1), P2, P3, P4, P6, P8, P10, P14, P16, P18, P20 and P30. The temporal bones were quickly removed and deep frozen in order to prepare cryosections for immunohistochemical staining with polyclonal antibodies against glucocorticoid receptors. The avidin-biotin, ABC-method was used to visualize binding. Both the vestibular and the cochlear regions of the inner ear were analysed. A faint staining of the crista ampullaris, the utricle and the cochlear duct was seen at E19; and staining became clearly visible at P1. A decrease in labelling was found at day 2-5 post partum whereafter an increased staining was again noticed until postnatal day 14 when an adult pattern was observed. The appearance of glucocorticoid receptors in the inner ear during development does not follow a linear curve. Further, the labelling pattern may indicate an impact of glucocorticoid receptors on the embryologic maturation itself as well as a functional role in the adult ear.
引用
收藏
页码:721 / 725
页数:5
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