Microwave-assisted solvent extraction -: a new method for isolation of polynuclear aromatic hydrocarbons from plants

被引：58

作者：

Tomaniová, M ^{[1
]}

Hajslová, J ^{[1
]}

Pavelka, J ^{[1
]}

Kocourek, V ^{[1
]}

Holadová, K ^{[1
]}

Klímová, I ^{[1
]}

机构：

[1] Inst Chem Technol, Fac Food & Biochem Technol, Dept Food Chem & Anal, CR-16628 Prague, Czech Republic

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 827卷 / 01期

关键词：

pollen; spruce needles; microwave-assisted solvent extraction; polynuclear aromatic hydrocarbons;

D O I：

10.1016/S0021-9673(98)00754-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The efficiencies of polynuclear aromatic hydrocarbons (PAHs) transfer into various solvents from pollen and spruce needles by two alternative extraction techniques - sonication and a microwave enhanced process - were compared. The effects of extraction mixture composition, temperature, time and number of repeated extractions on the recoveries were studied. Microwave extraction - realised at 140 degrees C for 20 min with n-hexane-acetone (3:2, v/v) as the extraction solvent was identified as the most effective extraction procedure for isolation of PAHs from biotic matrices (pollen and spruce needles). Gel permeation chromatography on Bio-Beads S-X3 with chloroform as a mobile phase was used for clean-up of extracts. HPLC with programmable fluorescence detection was employed for identification and quantification of analytes. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：21 / 29

页数：9

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[11] Heavy metal accessions to the forest floor of an urban forest reserve in Kuala Lumpur City, Malaysia [J].