Single-molecule quantum-dot fluorescence resonance energy transfer

被引：141

作者：

Hohng, S ^{[1
]}

Ha, T ^{[1
]}

机构：

[1] Univ Illinois, Dept Phys, Urbana, IL 61801 USA

来源：

CHEMPHYSCHEM | 2005年 / 6卷 / 05期

关键词：

DNA; fluorescence; FRET; quantum dots; single-molecule studies;

D O I：

10.1002/cphc.200400557

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Colloidal semiconductor quantum dots are promising for single-molecule biological imaging due to their outstanding brightness and photostability. As a proof of concept for single-molecule fluorescence resonance energy transfer (FRET) applications, we measured FRET between a single quantum dot and a single organic fluorophore Cy5. DNA Holliday junction dynamics measured with the quantum dot/Cy5 pair are identical to those obtained with the conventional Cy3/Cy5 pair, that is, conformational changes of individual molecules con be observed by using the quantum dot as the donor.

引用

收藏

页码：956 / 960

页数：5

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