Single-molecule quantum-dot fluorescence resonance energy transfer

被引:141
作者
Hohng, S [1 ]
Ha, T [1 ]
机构
[1] Univ Illinois, Dept Phys, Urbana, IL 61801 USA
关键词
DNA; fluorescence; FRET; quantum dots; single-molecule studies;
D O I
10.1002/cphc.200400557
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Colloidal semiconductor quantum dots are promising for single-molecule biological imaging due to their outstanding brightness and photostability. As a proof of concept for single-molecule fluorescence resonance energy transfer (FRET) applications, we measured FRET between a single quantum dot and a single organic fluorophore Cy5. DNA Holliday junction dynamics measured with the quantum dot/Cy5 pair are identical to those obtained with the conventional Cy3/Cy5 pair, that is, conformational changes of individual molecules con be observed by using the quantum dot as the donor.
引用
收藏
页码:956 / 960
页数:5
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