Determination of lincomycin and tylosin residues in honey using solid-phase extraction and liquid chromatography-atmospheric pressure chemical ionization mass spectrometry

被引：57

作者：

Thompson, TS

Noot, DK

Calvert, J

Pernal, SF

机构：

[1] Alberta Dept Agr, Food & Rural Dev, Food Safety Div, Agri Food Labs Branch, Edmonton, AB T6H 4P2, Canada

[2] Agr & Agri Food Canada, Beaverlodge, AB T0H 0C0, Canada

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 1020卷 / 02期

关键词：

honey; solid-phase extraction; American foulbrood disease; lincomycin; tylosin; antibiotics;

D O I：

10.1016/j.chroma.2003.08.095

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An analytical method for the determination of residues of the antibiotic drugs lincomycin and tylosin in honey was developed. The procedure employed a solid-phase extraction for the isolation of lincomycin and tylosin from diluted honey samples. The antibiotic residues were subsequently analyzed by reversed-phase HPLC with atmospheric pressure chemical ionization mass spectrometric detection. Average analyte recoveries for lincomycin and tylosin ranged from 84 to 107% in replicate sets of honey samples fortified with drug concentrations of 0.01, 0.5, and 10 mug/g. The method detection limits were determined to be 0.007 and 0.01 mug/g for lincomycin and tylosin, respectively. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：241 / 250

页数：10