Evaporative light scattering detection of pyrrolizidine alkaloids

被引：31

作者：

Schaneberg, BT

Molyneux, RJ

Khan, IA ^{[1
]}

机构：

[1] Univ Mississippi, Sch Pharm, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, University, MS 38677 USA

[2] Univ Mississippi, Dept Pharmacognosy, University, MS 38677 USA

[3] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA

来源：

PHYTOCHEMICAL ANALYSIS | 2004年 / 15卷 / 01期

关键词：

HPLC; evaporative light scattering detector; pyrrolizidine alkaloids; Senecio; Symphytum;

D O I：

10.1002/pca.715

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A reverse-phase high-performance liquid chromatography method utilizing evaporative light scattering detection (ELSD) has been developed for the simultaneous detection of hepatotoxic pyrrolizidine alkaloids with and without chromophores, namely, riddelliine, riddelliine N-oxide, senecionine, senecionine N-oxide, seneciphylline, retrorsine, integerrimine, lasiocarpine and heliotrine. Pyrrolizidine alkaloids were detected in five plant extracts (Senecio spartioides, S. douglasii var. longilobus, S. jacobaea, S. intergerrimus var. exaltatus and Symphytum officinale). The detection of heliotrine (which does not contain a chromophore) was much improved by ELSD compared with photodiode array detection. Copyright (C) 2004 John Wiley Sons, Ltd.

引用

页码：36 / 39

页数：4

共 14 条

[1]

[Anonymous], 2001, FDA ADV DIET SUPPL M

[2]

A GENERAL-METHOD FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PYRROLIZIDINE ALKALOID FREE BASES AND N-OXIDES [J].