Cytometry: Today's technology and tomorrow's horizons

被引：92

作者：

Chattopadhyay, Pratip K. ^{[1
]}

Roederer, Mario ^{[1
]}

机构：

[1] NIAID, ImmunoTechnol Sect, VRC, NIH, Bethesda, MD 20817 USA

来源：

METHODS | 2012年 / 57卷 / 03期

关键词：

Flow cytometry; Single-cell analysis; Data analysis; Polychromatic flow cytometry; Fluorescence reagents; POLYCHROMATIC FLOW-CYTOMETRY; T-CELL RESPONSES; IMMUNE; FOXP3; IDENTIFICATION; EXPRESSION; PROTECTION; PHENOTYPE; LINEAGE; SUBSETS;

D O I：

10.1016/j.ymeth.2012.02.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Flow cytometry has been the premier tool for single cell analysis since its invention in the 1960s. It has maintained this position through steady advances in technology and applications, becoming the main force behind interrogating the complexities of the immune system. Technology development was a three-pronged effort, including the hardware, reagents, and analysis algorithms to allow measurement of as many as 20 independent parameters on each cell, at tens of thousands of cells per second. In the coming years, cytometry technology will integrate with other techniques, such as transcriptomics, metabolomics, and so forth. Ongoing efforts are aimed at algorithms to analyse these aggregated datasaets over large numbers of samples. Here we review the development efforts heralding the next stage of flow cytometry. Published by Elsevier Inc.

引用

页码：251 / 258

页数：8

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